ORIGINAL ARTICLE Integration of anti-penetrant tricyclazole, signaling molecule salicylic acid and root associated Pseudomonas fluorescens enhances suppression of Bipolaris sorokiniana in bread wheat (Triticum aestivum L.) Udai B. Singh 1 & Shailendra Singh 1 & Deepti Malviya 1 & Nanjappan Karthikeyan 1 & Mohd. Imran 2 & Rajan Chaurasia 3 & Manzar Alam 2 & Pratap Singh 4 & B. K. Sarma 5 & Jai P. Rai 5 & T. Damodaran 6 & J. K. Tripathi 7 & Sunil Kumar 1 & Arun K. Sharma 1 Received: 16 April 2018 /Accepted: 19 March 2019 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019 Abstract Salicylic acid (SA) and root associated Pseudomonas fluorescens are known inducers of systemic resistance and influence plant defense responses including reprogramming of cellular mechanisms in response to biotic stress. However, integration of SA, tricyclazole and bioagent P. fluorescens in the integrated disease management practices is not clearly understood. With this rationale the present study was carried out to understand the effects of integration of an anti-penetrant tricyclazole, signaling molecule SA and P. fluorescens UBSPF-10 in modulation of defense cascades and enhancing suppression of Bipolaris sorokiniana in bread wheat (Triticum aestivum L.). Results showed that exogenous application of SA (100 mg L -1 ) alone and in combination with tricyclazole (750 mg L -1 ) and P. fluorescens UBSPF-10 significantly increased activation and accumulation of phenylalanine ammonia lyase (PAL), peroxidase, β 1,3-glucanase and chitinase activities in wheat leaves challenged with B. sorokiniana compared to individually treated and control plants. The influence of combined applications of SA, tricyclazole and P. fluorescens UBSPF-10 on lignin content in plant leaves was investigated and it was observed that the combined treatment resulted significant increase in the acid-soluble lignin content in wheat possibly leading to enhanced suppression of disease progression and severity. Furthermore, correlation analysis clearly indicated that enhanced accumulation of lignin in plant cells significantly reduces pathogen infection and invasion of tissue leading to reduction in disease development compared to other treatments. Therefore, it is concluded that combined application of SA, tricyclazole and P. fluorescens UBSPF-10 played an important role in suppression of B. sorokiniana development in wheat plants in a cooperative manner and thus, could be used in the integrated disease management practices of wheat. Keywords Salicylic acid . Pseudomonas fluorescens . Biotic stress . Triticum aestivum . Bipolaris sorokiniana . Induced systemic resistance * Udai B. Singh udaiars.nbaim@gmail.com; nbaimudai@gmail.com 1 Plant-Microbe Interaction and Rhizosphere Biology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan 275 103, India 2 Department of Biosciences, Faculty of Science, Integral University, Lucknow 226 026, India 3 Department of Botany, Faculty of Sciences, Banaras Hindu University, Varanasi 221 005, India 4 Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India 5 Department of Mycology & Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India 6 Regional Research Station, ICAR-Central Soil Salinity Research Institute, Old Jail Road, Near Manyawar Kanshiram Smarak Sthal, Lucknow, India 7 ICAR-Indian Institute of Seed Sciences, Kushmaur, Maunath Bhanjan 275 103, India Journal of Plant Pathology https://doi.org/10.1007/s42161-019-00296-5