Int.J.Curr.Microbiol.App.Sci (2020) 9(2): 734-739 734 Original Research Article https://doi.org/10.20546/ijcmas.2020.902.090 Influence of Indigenous Nicobari and Ankleshwar Chicken Breeds on Physical and Biochemical Attributes of Semen V. Beulah Pearlin * , J. Mohan, J. S. Tyagi, M. Gopi, G. Prabakar and M. Shanmathy ICAR-Central Avian Research Institute, Izatnagar- 243 122, Uttar Pradesh, India *Corresponding author ABSTRACT Introduction For the success of artificial insemination in poultry, examination of the semen characteristics of different breeds of poultry and its preservation is the need of the day. The evaluation of semen characteristics emulates the reproductive potential of the cock and hence the major determinant of fertility and subsequent hatchability of eggs (Zahraddeen et al., 2005). Such types of studies are lacking in indigenous chicken breeds such as Nicobari and Ankleshwar. Nicobari fowl is an endangered and endemic breed of poultry in the islands of Andaman and Nicobar and produces highest number of eggs among all the indigenous chicken breeds of India (Ahlawat and Chatterjee, 2002). Ankleshwar breed of chicken belong to the ‘Ankleshwar’ area of Bharuch district of Gujarat and are reared mainly by the tribal population under backyard poultry farming as International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 2 (2020) Journal homepage: http://www.ijcmas.com The objective of the current experiment was to investigate the various semen characteristics of two indigenous breeds of chicken namely Nicobari and Ankleshwar, as such data were not yet reported. Freshly ejaculated semen samples were collected from twenty breeder roosters of each breed and subjected to physical and biochemical analysis. Immediately after collection, the semen volume of individual birds was recorded using tuberculin syringe and further analysed for seminal motility, concentration and percentage of live, dead and abnormal spermatozoa using standard procedures. After recording the hydrogen ion concentration in semen, other biochemical attributes such as total protein, triglyceride, cholesterol, transaminase enzymes, alkaline phosphatase, calcium, phosphorus and uric acid concentration were estimated in the seminal plasma of both the native breeds. The physical characterization of semen showed significant (p<0.05) breed differences in seminal volume and per cent morphologically abnormal spermatozoa. Significant (p<0.05) biochemical changes were seen in the concentrations of triglyceride, cholesterol, Glutamic oxaloacetic transaminase and uric acid. The study showed significant breed variations in the seminal attributes of the selected native breeds of chicken. Keywords Ankleshwar, Biochemical, Indigenous, Nicobari, Physical, Semen Accepted: 08 January 2020 Available Online: 10 February 2020 Article Info