~ 73 ~ Journal of Pharmacognosy and Phytochemistry 2020; SP6: 73-75 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2020; SP6: 73-75 Devesh Kumar Pandey Faculty of Agricultural Science, CP University Kota Rajasthan Krishna Kumar Singh HNB Garhwal University, Srinagar (Garhwal), Uttrakhand, India Adarsh Pandey Faculty of Agricultural Science and Allied Industries at Rama University, Kanpur (U.P.), India Alok Kumar Pandey Faculty of Agricultural Science CP University Kota Rajasthan, India Correspondence Devesh Kumar Pandey Faculty of Agricultural Science CP University Kota Rajasthan, India International Web-Conference On New Trends in Agriculture, Environmental & Biological Sciences for Inclusive Development (21-22 June, 2020) Effect of bio-fertilizer on flowering, and corm parameters of Gladiolus cv. American beauty Devesh Kumar Pandey, Krishna Kumar Singh, Adarsh Pandey and Alok Kumar Pandey Abstract An experiment was undertaken to explore the effect on flowering and corm parameters of the biofertilizer in gladiolus (Gladiolus grandiflorus L.) cv. and laid out in Randomized Block Architecture (RBD) experiment with Azotobacter, Phosphobacteria, Azospirllium (Azotobacter + Phosphobacteria), respectively, (Azospirillum + Phosphobacteria). Results showed that minimum days taken to spike initiation (48.67 days), maximum diameter of 2nd floret (9.14 cm), number of florets per spike (10.86), number of spikes per plant (2.90), number of spikes per hectare (398065.70), number of corms per plant (2.59), weight of corms per plant (74.00 g), weight of cormels per plant (11.92 g), size of corm (5.86 cm), nitrogen (1.84 percentages per plant) Bio-fertilizer was identified in this experiment, in order to increase soil fertility and crop production in sustainable agriculture. Keywords: Bio-fertilizer, plant growth, flower quality, nutrient uptake, Gladiolus Introduction Gladiolus (Gladiolus grandiflorus L.) is one of the most common ornamental plants cultivated for its enchanting flowers in many parts of the world. It is renowned internationally for its spectacular florets of colour, robust spike, scale, elegant appearance and consistency that occupies fifth place in international trade. It is native to South Africa, and ranks second in the Netherlands market among the bulbous cut flowers. This crop’s success as a cut flower is increasing worldwide, which is a possible money-spinner for the floriculture industry with annual production of about 120 million spikes. To ensure productivity maximisation in any crop nitrogen, phosphorus and potassium are the three main nutrients that play a very crucial role in influencing vegetative growth, flower yield and quality attributes. However, given the recent definition of eco-friendly production increased prices, and the timely non-availability of inorganic fertilisers, the use of chemicals contributing to low soil fertility and soil quality is selective. The use of cost-effective and eco-friendly bio-fertilizers in recent years has resulted in improved production of many crops, of addition to increasing soil quality and fertility levels along with preserving nutrient physical properties. Various bio-fertilizers, namely Azotobacter and Trichoderma are significant. Azotobacter is a symbiotic bacterium where it lives in tandem with the host and fixes nitrogen in the environment. The symbiotic combination of higher plant fungi and root systems called "Mycorrhiza." Bio-fertilizers have a supplementary nutritional function in and by their use in productivity. The various agro-techniques and nutritional requirements need to be streamlined by including nutrient management method for productivity improvement and gladiolus spike consistency. A field investigation was performed to research the impact of biofertilizer on parameters of gladiolus cv in flowering and corm. American conditions for beautyunder open area. Material and methods The experiment was carried out at the School of Agriculture, Research Farm, Career Point University, Kota, Rajasthan, India. And laid out in Randomized Block Design replicated thrice with three treatments.