51 The Infuence of Administering Propolis CMCE Extract on FSH, LH, and Testosterone Levels ... • pISSN: 2085-1545 • eISSN: 2339-093X http://jurnal.unissula.ac.id/index.php/sainsmedika Sains Medika, Vol. 10, No. 2, July - December 2019 : 51-56 Copyright @ 2019 Authors. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial- ShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are properly cited. RESEARCH ARTICLE ABSTRACT Introduction: Monosodium Glutamate (MSG) increases free radicals and affects male reproductive hormone balance. Continuous Multi-Stage Countercurrent Extraction (CMCE) propolis extract is often used as supplement to prevent cellular damage induced by free radical. Objective: Discover the effect of administering CMCE propolis extract to increase follicle stimulating hormone (FSH), luteinizing hormone (LH) and Testosterone (Te) levels in MSG-induced male Wistar rats. Methods: This research is an experimental research with post-test only control group design using 18 male Wistar rats as its sample, divided into 3 groups. The control group (C-G) was given MSG 140mg/day and received no propolis CMCE extract. Propolis groups 8.3 (CP-83) and 10.8 (CP-108) also given MSG 140mg/day, and each received propolis CMCE extract 8.3 mg and 10.8 mg/200grBW/day. All treatments were provided orally for 21 days. On the 22nd day, their bloods were taken through orbital sinus to have their FSH, LH and Te levels measured using ELISA. The obtained data were analyzed using ANOVA test and followed with Post Hoc LSD test. Results: The ANOVA analysis indicated that significant differences occurred in FSH, LH, and Te levels among the groups, p<0.001. The Post hoc analysis showed that the FSH, LH and Te levels in CP-83 and CP-108 groups were significantly higher than group C-G, p<0.001. The FSH, LH and Te levels in CP-108 group was significantly higher than group CP-83, p<0.001. Conclusion: Administering propolis CMCE extract at 8.3 and 10.8 mg/day could increase the FSH, LH and Testosterone levels in MSG-induced male Wistar rats. Keywords : CMCE propolis extract, FSH, LH, Testosteron, Monosodium glutamate ABSTRAK Pendahuluan: Pemberian Monosodium glutamate (MSG) telah terbukti dapat meningkatan radikal bebas dan berpengaruh terhadap keseimbangan hormon reproduksi pria. Ekstrak Continous Multi-Stage Countercurrent Extraction (CMCE) propolis sering digunakan untuk suplemen dalam menangkal radikal bebas dari luar. Tujuan: Mengetahui pengaruh pemberian Ekstrak CMCE propolis terhadap peningkatan kadar Follicle stimulating hormone (FSH), Luteinizing hormone (LH) dan Testosteron (Te) pada tikus Wistar jantan yang diinduksi MSG. Metode: Penelitian eksperimental dengan post test only control group design menggunakan sampel 18 ekor tikus Wistar jantan, dibagi menjadi 3 kelompok. Kelompok Kontrol (C-G) diberi MSG 140mg/hari dan tidak mendapatkan ekstrak CMCE propolis. Kelompok propolis 8.3 (CP-83) dan 10.8 (CP-108) selain diberi MSG 140mg/hari, masing masing juga mendapatkan ekstrak CMCE propolis 8.3mg dan 10.8mg/200grBB/hari. semua perlakuan diberikan secara oral selama 21 hari. Hari ke-22 dilakukan pengambilan darah melalui sinus orbitalis kemudian diukur kadar FSH, LH dan Te dengan metode ELISA. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan uji Post Hoc LSD. Hasil: Uji analisis anova menunjukan terjadi perbedaan bermakna kadar FSH, LH, dan Te di antara kelompok, p<0,001. Analisis Post hoc menunjukan bahwa kadar FSH, LH dan Te pada kelompok CP-83 dan CP-108 lebih tinggi bermakna dibanding kelompok C-G, p<0,001. Kadar FSH, LH dan Te pada kelompok CP-108 lebih tinggi secara signifikan dibandingkan kelompok CP-83, p<0,001. Kesimpulan: Pemberian ekstrak CMCE propolis dengan dosis 8.3 dan 10.8mg/hari dapat meningkatkan kadar FSH, LH dan Testosteron pada tikus Wistar jantan yang diinduksi MSG. Kata Kunci: ekstrak CMCE propolis, FSH, LH, Testosteron, Monosodium glutamate INTRODUCTION Infertility can be defined as the inability to achieve pregnancy after sexual intercourse for 12 months with no protection (Practice commitee of American Society for Reproductive Medicine, 2013). Infertility can be caused by various factors, neither from the male nor the female partners. The factors from male partners contribute to forty percent of infertile couples (World Health Organization (WHO), 2010). One of the causes of infertility in men is oxidative stress resulting from increased Reactive Oxygen Species (ROS) level (Ko, Sabanegh and Agarwal, 2014). The evidence shows that The Influence of Administering Propolis CMCE Extract on FSH, LH, and Testosterone Levels in MSG-Induced Male Wistar Rat Eko Naning Sofyanita 1* , Taufiqurrachman Nasihun 1 , Siti Thomas Zulaikhah 1 1 Program Studi Ilmu Biomedik, Fakultas Kedokteran, Universitas Islam Sultan Agung Semarang Jl. Kaligawe KM 4 Semarang 50012 Telp (+6224) 6583584 Fax (+6224) 6594366 Semarang *Corresponding author email: en.sofyanita@gmail.com +6281353439026