1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 z Organic & Supramolecular Chemistry Metal-Free C-H Thiomethylation of Quinones Using Iodine and DMSO and Study of Antibacterial Activity Sakthidevi Rajasekar + , [a] T. P. Adarsh Krishna + , [a] Nagendran Tharmalingam + , [b] Ilangovan Andivelu,* [a] and Eleftherios Mylonakis* [b] A novel, transition metal-free, molecular iodine mediated method for thiomethylation of quinones, using dimethyl sulfoxide (DMSO) as a benign thiomethylating agent is described. This greener reaction protocol leads to selective mono thiomethylation in low to high yield and tolerates a range of substituent groups. Preliminary antibacterial evalua- tion of thiomethylated quinones, carried out against methicillin resistant Staphylococcus aureus shows promising results. One of the compound 3-methyl-6-(methylthio)-[1,1-biphenyl]-2,5-di- one exhibited antimicrobial activity even against clinical isolates of S. aureus and vancomycin-intermediate Staphylococ- cus aureus (VISA). Introduction Quinone, an interesting structural motif with unique electronic properties [1] is found among natural products, [2] terpenequinones, [3] kinamycin antibiotics [4] and organic materials. [5] Although quinone was functionalised earlier using pre-functionalized starting materials, [6] direct C H functionaliza- tion of quinones was reported later mainly using expensive transition metal catalysts like Pd, Ru and also inexpensive iron salts. [7a–c] Nevertheless, development of metal free [7d–f] simple method for direct C–H functionalization of quinones still remains a challenge. Over the past few years, iodine has been explored [8] as a green alternative to transition metal catalysts in organic synthesis, as iodine has diverse valence states as well as moderate redox potentials. [9] Having initiated by Ishihara and Wan, iodine has been used in carbodehydrogenative coupling reactions for the formation of C–O, C–N and C–C bonds. [10] Li and co-workers used I 2 as an alternative to transition metal catalyst in oxidative cyclization of N-aryl enamines to indoles. [9d] Thioether is an important functional group found among drugs, [11] novel synthetic building blocks, [12–13] materials [14a–c] and 2-alkyl and 2-arylthio-1,4-naphthaquinones which shows cho- lestrol acyltransferase inhibition activity. [14d–e] Construction of thioethers by addition of sulfur or sulfonyl radicals to C C multiple bonds is a common strategy. [15] Conventional method for preparation of thiomethyl ethers consists of directed or heteroatom-facilitated lithiation and subsequent electrophilic substitution with dimethyl disulfide. [16] An alternate approach is, chlorosulfonylation of electron-rich rings using chlorosul- fonic acid followed by reduction and then S-methylation with MeI. [17] Several copper promoted C–H thiomethylation methods have emerged. 1819 These include thiomethylation of 2-phenyl- pyridine using MeSSMe Cu(OAc) 2, [20] arenes and hetero arenes [21a] using DMSO Cu(OAc) 2 , and 2-arylpyridine using DMSO-CuF 2 (4 equiv.). [21b] A new copper catalyst system for thiolation of heteroarenes [21c] regioselective sulfencataylation of flavones was also reported. [21d] However, the main limitation of these transformations are the need for the presence of a chelating hetero atom, and limited substrate scope. Interest- ingly, iodine was used in promotion of annulation followed by thiomethylation in construction of 3-(methylthio)-4-aryl-1H- pyrrole-2,5-diones, [21e] and 6-iodo-3-(methylthio)-2-arylimidazo [1,2-a]pyridines. [21f] Although, transition metal catalyzed carbon-sulfur bond forming reactions [22] on different class of compounds are well established, methods for preparation alkylthioquinones are only very few. [14d–e] During the course of writing this manuscript, Poulsen et al reported, as a part of study on heterofunctional- ization, thioethylation of quinones using odorous EtSH and Co (OAc) 2 catalyst. The yield is good to low and only three examples were studied and bisthioethylated products were formed. [14f] Thus an important drawback of thioalkylation of quinones was the need for use of thiols, which are hazardous, unstable to air, moisture sensitive and possess unpleasant odor. Further, the hydroquinone formed in situ, should be re- oxidized to quinone using a strong and highly toxic oxidant like chromates. Additionally, only an isolated example on formation of 2-(methylthio)-naphthoquinone from 1-naphthol was reported. [23] Thioether derivatives are good antibacterial agents. For example, pleuromutilin derivatives, substituted with thioether group at the C14 side chain, show activity against Staph- ylococcus aureus strain ATCC26112, [24a] and 1,3,4-oxadiazole [a] Dr. S. Rajasekar, + T. P. A. Krishna, + Prof. I. Andivelu School of Chemistry, Bharathidasan University, Tiruchirappalli, Tamilnadu- 620024, India E-mail: ilangovanbdu@yahoo.com [b] Dr. N. Tharmalingam, + Prof. E. Mylonakis Infectious Diseases Division, Warren Alpert Medical School of Brown University, Rhode Island Hospital, Providance, RI 02903, USA E-mail: emylonakis@lifespan.org [ + ] Authors contributed equally Supporting information for this article is available on the WWW under https://doi.org/10.1002/slct.201803816 Full Papers DOI: 10.1002/slct.201803816 2281 ChemistrySelect 2019, 4, 2281–2287 © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim