Vol.:(0123456789) 1 3
Environmental Sustainability
https://doi.org/10.1007/s42398-018-0024-0
REVIEW
Iron oxidizing bacteria: insights on diversity, mechanism of iron
oxidation and role in management of metal pollution
Vipin Kumar Singh
1
· Asha Lata Singh
1
· Rishikesh Singh
2
· Ajay Kumar
1
Received: 30 April 2018 / Revised: 13 August 2018 / Accepted: 17 August 2018
© Society for Environmental Sustainability 2018
Abstract
In natural ecosystems, diverse iron oxidizing bacteria are of common occurrence. Basically, two diferent mechanisms have
been proposed for catalysis of iron oxidation by bacterial metabolic systems which difer mainly at cytochrome and rusti-
cyanin level. Biological iron oxidizers not only afect the cycling of iron but also efciently minimize the concentrations of
hazardous metals such as lead, nickel, copper, chromium, cadmium and cobalt. The ferric iron generated after biological
oxidation forms complexes with metals/metalloids present in their vicinity. Ferric ions produced by biological actions also
act as catalyst for oxidation of toxic metalloid such as arsenite (As III) converting it into less toxic form. Most importantly,
bacterial iron oxidizers have commercially been employed in industrial bioleaching for the recovery of important elements
and remediation of acid mine drainage water. Currently, heavy metal contamination has emerged as one of the prime con-
cerns for the world and is posing serious threats to both environment and human health. Although varieties of physical and
chemical techniques are currently being used to manage the metal contamination, treatment using biological iron oxidation
approaches are convincing because of their ecofriendly nature and low sludge generation. In the present review we have tried
to focus on the diversity of bacterial iron oxidizers, mechanisms of iron oxidation by bacterial species, and role of bacterial
iron oxidizers in bioremediation of metal pollutants along with future research possibilities in this area.
Keywords Iron oxidizers · Arsenic · Bioremediation · Metal contamination · Ferric iron · Sludge
Introduction
Iron is one of the abundantly occurring elements on the
Earth (Wedepohl 1995) and crucial for multiple biologi-
cal activities. A number of oxidation states have been pro-
posed for iron, but +2 (ferrous) and +3 (ferric) forms are
the most common. The changes in oxidation number are
afected by environmental conditions like pH, oxygen con-
tent, and oxidation reduction potential. Since conversion of
ferrous iron to ferric iron is highly oxygen dependent, we
can expect the presence of ferrous iron, and therefore iron
oxidizing bacteria, only under anoxic environments such as
deep ground water surfaces or under highly acidic condition
where oxidation is negligible to very slow (Bird et al. 2011).
Under these oxygen depleted conditions, iron oxidizing
bacteria utilize ferrous iron for oxidation to accomplish the
energy requirement for carbon assimilation (Schwertmann
and Cornell 2000). Among various types of iron oxidizers,
bacterial iron oxidizers are widely described. Most of the
bacterial iron oxidizers belong to phylum Proteobacteria.
The bacterial genera performing the oxidation may be auto-
trophic, heterotrophic, phototrophic, chemotrophic, aerobic
and anaerobic (Bird et al. 2011). Their huge diversity in
physiology and phylogeny has made them a good tool for
innovative research in the area of pollution management.
Iron oxidizers are the important players not only in global
iron biogeochemical cycling (Bach and Edwards 2003) but
also in cycling of other toxic metals and industrial biomin-
ing activities (Rawlings and Johnson 2007). Therefore, there
is growing attention amongst researchers on iron oxidation
process, genetic regulation of iron oxidation (Bird et al.
2011), industrial application (Zhang et al. 2018), their phylo-
genetic relationship, and diversity along with their adaptive
* Vipin Kumar Singh
vipinks85@gmail.com
1
Department of Botany, Centre of Advanced Study
(CAS), Institute of Science, Banaras Hindu University,
Varanasi 221005, India
2
Institute of Environment and Sustainable Development,
Banaras Hindu University, Varanasi 221005, India