Regul. Mech. Biosyst., 9(3) Regulatory Mechanisms in Biosystems ISSN 2519-8521 (Print) ISSN 2520-2588 (Online) Regul. Mech. Biosyst., 9(3), 340–346 doi: 10.15421/021850 Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties T. E. Talankova-Sereda*, J. V. Kolomiets*, A. F. Likhanov**, A. V. Sereda***, N. I. Kucenko****, E. O. Shkopinskiy***** *National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine **Institute for Evolutionary Ecology of NAS of Ukraine, Kyiv, Ukraine ***Chinese-Ukrainian Life Sciences Research Institute, Zhuji City, China ****Experimental Station of Medicinal Plants at the Institute of Agroecology and Nature Management of NAAS, Beresotocha, Ukraine *****Zaporizhzhia National University, Zaporizhzhia, Ukraine Article info Received 14.06.2018 Received in revised form 20.07.2018 Accepted 28.07.2018 National University of Life and Environmental Sciences of Ukraine, Heneral Rodimtsev st., 19/4, Kyiv, 03041, Ukraine. Institute for Evolutionary Ecology NAS Ukraine, Lebedev st., 37, Kyiv, 03143,Ukraine. Sino-Ukrainian Institute of Life Sciences, South Wenzhou st., 26, Zhuji City, Prov. Zhejiang, Republic of China. Experimental Station of Medicinal Plants at the Institute of Agroecology and Nature Management of NAAS, Lenin ave., 16a, Beresotocha, 37535, Ukraine. Zaporizhzhia National University, Zhukovskogo st., 66, Zaporizhzhya, 69600, Ukraine. Tel.: +38-050-983-58-53. E-mail: tatazp77@gmail.com Talankova-Sereda, T. E., Kolomiets, J. V., Likhanov, A. F., Sereda, A. V., Kucenko, N. I., & Shkopinskiy, E. O. (2018). Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties. Regulatory Mechanisms in Biosystems, 9(3), 340–346. doi:10.15421/021850 Quantitative and qualitative composition of essential oils of peppermint breeds Lebedinaya Pesnya, Lubenchanka, Lidiya, Ukrainskaya Perechnaya, Mama, Chornolista was investigated before and after clonal microreproduction by the method of isolated tissues and bodies culture in vitro. Methods of essential oil steam distillation, capillary gas chromatography and statistical analysis were used in the research. It is established that increase in essential oil quantity was observed for peppermint breeds on which reproduction and improvement іn vіtro technology was applied. As a result of clonal microreproduction of peppermint plants in culture іn vіtro on nutrient medium Murasige and Skug, in which the growth regulators 0.75 mg/l of 6- benzylaminopurine, 0.1 mg/l of adenine, 0.05 mg/l of indolil-3-acetic acid and 0.5 mg/l of gibberellins acid were added and virocide Ribavirin in concentration 10 mg/l, improvement was obtained in comparison with vegetatively reproduced plants; increase in essential oil quantity per hectare was established for the following breeds; Chornolista by 54.2%, Lebedinaya pesnya by 38.2%, Ukrainskaya Perechnaya by 36.7%, Mama by 28.5%, Lubenchanka by 17.1% and Lidiya by 11.6%. For oil content the highest indices were noted for Lubenchanka, Mama and Lebedinaya Pesnya peppermint breeds with product yield 4.02%, 3.98% and 3.84% respectively. It was established that the essential oil component composition in non-clonal peppermint plants raw materials and plants-regenerants after culture in vitro is variable depending on breed. Limonene, cineole, menthone, menthofuran, iso- menthone, menthyl acetate, β -caryophyllene, iso-menthol, menthol, pulegone, germacren, piperitone, carvone were identified in peppermint essential oil. High content of menthol, low content of carvone, piperitone, pulegone (except for Chornolista, Ukrainskaya Perechnaya breeds) and menthofuran (except for Chornolista, Ukrainskaya Perechnaya and Lubenchanka breeds) are characteristic for Ukrainian selection peppermint investigated breeds. A clear tendency to menthol and menthone content ratio increase is observed in plants which were improved in conditions іn vіtro. Pulegone was not detected in essential oil samples of Lebedinaya Pesnya, Lidiya and Mama breeds. Biochemical markers of Lebedinaya Pesnya, Lubenchanka, Mama breeds, which differentiate them within the group of investigated breeds, are higher limonene, piperitone and menthol pool; for Ukrainskaya Perechnaya and Chornolista breeds – pulegone, cineole and menthone; for Lidiya breed – iso-menthone. Keywords: Mentha pіperіta; іn vіtro; capillary gas chromatography; menthol; menthone; pulegone. Introduction According to State Statistics Service data nearly 50 species of plants whose raw materials are used for medicinal preparation manufac- ture are cultivated in Ukraine. Structural parts of these plants contain biologically active substances which positively influence the human organism. Peppermint is one of the most widespread and traditionally cultivated species. It is cultivated as industrial culture in all climatic zones of Ukraine, Moldova, Russia (Krasnodar territory and North Caucasus), Belarus, and also in many countries of Europe, Central Asia, Africa, India and the North America. Such soil-climatic variety of cultivation zones testifies to the considerable adaptive potential of this culture. The demand for medicinal preparations on the basis of natural vegetative raw materials has increased in the last 10–15 years and the Ukraine home market does not satisfy the needs of the domestic chemical-pharmaceutical industry, which requires every year nearby 50 tonnes of M. pіperіta essential oil and 20–25 tonnes of natural menthol (Shelud'ko, 2004). Mint is a source for obtaining pharmaceutic leaf, essential oil and its components, which are widely used in the chemical-pharmaceutical, perfume-cosmetic, confectionery, food, liqueur and spirits, tobacco, paint and coatings industries and medicine (Vojtke- vіch, 1999; Tkachenko, 2011). The biologically active substances of peppermint are complex and its essential oil shows anti-inflammatory, antimicrobial, antifungal, anti- viral, anthelmintic, antiparasitic, analgetic, spasmolytic, cholagogic, mu- colytic and broncholytic, antihistaminic action, normalises central and vegetative nervous system activity, limits rotting and fermentation processes, strengthens motor secretory intestinal tract function, prevents peptic ulcer development, expands coronary vessels, improves heart blood supply, reduces pressure in blood circulation small circle, and also shows 340