Review: Direct conversion in Neuro-regenerative
Medicine
Myung Rae Park
1
, Danbi Song
1
, Jihye Park
1
, Kang Seok Lee
1
, Suk Hwan Joung
1
,
Jeong Beom Kim
1*
1
Max Planck Partner Group-Molecular Biomedicine Laboratory, School of Life Sciences, Ulsan
National Institute of Science and Technology, Ulsan, South Korea
Abstract. Recent advances in direct conversion technology are of great
significance in neuro-regenerative medicine. Conversion of somatic
cells to a desired cell type is next generation reprogramming technology
for clinical applications. The novel strategy of direct conversion of
somatic cells into expandable multipotent neural cells (induced
multipotent neural cells, iMNCs) and specific neurons (induced neurons,
iNs) opens a new window of opportunities for cell therapy, disease
specific modeling, and drug discovery for brain diseases.
Keywords: Direct conversion, multipotent neural cells, somatic cells,
neurodegenerative disorders
1 Introduction
Regenerative medicine opens new windows for effective and safe therapies for
incurable and debilitating disorders. Stem cells, which can be isolated from human
tissues, are considered as an essential resource in regenerative medicine. There are
several types of stem cells such as embryonic stem cells (ESCs), adult stem cells, and
induced pluripotent stem cells (iPSCs) depending on their developmental sources [1].
The discovery of iPSCs in 2006 not only offered an opportunity for a patient-specific
cell-based therapy but also provided disease-specific modeling, which can be used as
a valuable platform for drug screening. Furthermore, the derivations of iPSCs from
adult cells avoided the ethical debate concerning the derivation of ESCs from human
embryos [2]. However, many practical obstacles still remain to bring stem cell
technology from bench to bed. One of the limitations is difficulty in inducing
differentiation of pluripotent stem cells into a desired cell type. It takes significant
time and efforts to reprogram somatic cells to pluripotent cells and later induce them
into desired cells. Another limitation is that there still is a high possibility that
remaining undifferentiated cells may form a teratoma during differentiation within the
human body. To date, there are many reviews on induced conversion of specialized
cells into other cell types by using lineage specific transcription factors. In the past
few years, many research groups have focused on direct conversion between somatic
cells, within or outside their original lineages [3]. Neurodegenerative diseases such as
Parkinson’s disease (PD), Alzheimer’s disease (AD), Amyotrophic Lateral Sclerosis
(ALS) and Multiple Sclerosis (MS) occur as the result of neurodegenerative processes
Advanced Science and Technology Letters
Vol.47 (Bioscience and Medical Research 2014), pp.79-83
http://dx.doi.org/10.14257/astl.2014.47.19
ISSN: 2287-1233 ASTL
Copyright © 2014 SERSC