Pakistan J. Zool., vol. 37(3), pp. 169-174, 2005. Spiders of the Genus Clubiona (Araneae : Clubionidae) from Punjab, Pakistan MUHAMMAD KHALID MUKHTAR AND SHAKILA MUSHTAQ Araneae Laboratory Department of Zoology and Fisheries, University of Agiculture, Faisalabad. Email: mkmukhtar@yahoo.com and mkmukhtar@hotmail.com Abstract.- Spider fauna of the genus Clubiona was surveyed during 1996 to 1998 and 2000 from 25 collection sites of 19 districts of the province of Punjab. Collection was done by jarring and handpicking from the foliage of 26 different host plants including forest plantations, crops, and orchards, ornamental and wild plants. In all, 240 specimens (99 females, 62 males and 79 Imm) were captured representing five species. Of these one species (Clubiona kasurensis) is new to science and one species (Clubiona filicata, O. P.-Cambridge) new record for Pakistan. A key to the species so for recorded from Pakistan has been developed. Key words: Clubiona, news species, new record, Pakistan. INTRODUCTION The Clubiona, Latreille is a very large genus with 429 known species all over the world (Platnick, 2004).The genus has been studied from Asia by several workers. Gravely (1931), Tikader and Biswas (1981), Majumder and Tikader (1991) Biswas and Biswas (1992), Biswas and Majumder (1995), Barrion and Litsinger (1995), Biswas and Raychaudhuri (1996), Zhu and An (1999), Song et al. (1999), Hu (2001), and Patel (2003) made significant contribution from India, Bangladesh, China, and South and Southeast Asia. It has been studied in Pakistan by Qadir (1997) from Sialkot, Nazir (1999) from Bahawalpur, and Nazir (2001) from Jhang but there work is unpublished. This paper reports a total of five species including a new species and a new record in the collection of 240 specimens (99 females, 62 males and 79 Imm). MATERIALS AND METHODS The survey was carried out during 1996-1998 and 2000 in 25 collection sites from 19 districts of the province of the Punjab. Collection was done by jarring and handpicking from the foliage of 26 different host plants including forest plantations, crops, orchards and ornamental and wild plants. The specimens were transferred into a container. The 0030-9923/2005/0003-0169 $ 8.00/0 Copyright 2005 Zoological Society of Pakistan. having 70% ethyl alcohol, before being brought to the laboratory. Collected specimens were washed with xylene. Each specimen was preserved in a separate vial in a mixture of 1:1 ethyl alcohol and glycerine. Permanent slides of the genetalia were prepared by KOH treatment followed by dehydration, clearing in clove oil and mounting in Canada Balsam. Epigynes were carefully removed from the abdomen by lifting the mid epigastric furrow using insect needle. Male palpi were removed from the base using the same needle and mounted in Canada Balsam after treatment with xylene. Identification was done on the basis of morphological characters of various body parts using literature mentioned in the introduction and Nentwig et al. (2003). A comprehensive description of only the new species has been given. However, the already known species which came into collection have been provided with present and previous locality records. Measurements (mm) of various body parts of the specimens were taken with the help of ocular micrometer. Ranges of various body parts (except legs) with their means and standard deviations of the new species are also given. The leg measurements were given in the following sequence: total (femur, patella + tibia, metatarsus, tarsus). The spinnation on the legs has been represented in this sequence: dorsal, ventral, prolateral, and retrolateral following Barrion and Litsinger (1995). Drawings of important body parts of the new species were drawn with the help of ocular grid.