1 VOLUME 21 NO 01 2020, pp 01-06 ISSN : Print 1411-3724 — Online 2549-7464 DOI : https://doi.org/10.24036//eksakta/vol21-iss1/210 : Berkala Ilmiah Bidang MIPA Eksakta http://www.eksakta.ppj.unp.ac.id/index.php/eksakta Eksakta Berkala Ilmiah Bidang MIPA Article The Effect of Ethanol Solvent Concentration on Antimicrobial Activities The Extract of Andalas Endophytic Bacteria ( Morus Macroura Miq.) Fermentation Product Larasati Arum Utami 1 , Dwi Hilda Putri 1* Department of Biology, Faculty of Mathematics and Natural Science (FMIPA), Universitas Negeri Padang, Indonesia Abstract. Anti-Biotic resistence is a health problem globally. According to the data of WHO, there is an increasing resistance of Staphylococcus sp. bacterium toward anti-biotic to 80% in 2013. It is able to overcome with a new anti-microbial compound which can be produced from Andalas endophytic bacteria. This compound can be obtained through fermentation process. In order to separate this active-compound, it is needed to use extraction method. In this method, the solvent is functioned as the extractor. One of the solvent which is commonly used is ethanol. This research is aimed to know the effect ethanol concentration toward antibacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria isolate JDT 1B. The fermented products are extracted by using maceration method. The concentrations are 100%, 80%, 70%. A test of anti-microbial activity is used disk diffusion method. The extracted concentrations tested for each solvent are 50%, 25%, 12,5%, and 6.25%. Anti-bacterial activity is analyzed by using factorial design. The factorial result showed there is no significant contrast between ethanol concentration mentioned toward anti-bacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria Isolate JDT 1B. The concetration of extracted fermentation product using 70% ethanol has the same inhibition zone as control is 6,25%. . This is an open acces article under the CC-BY license. This is an open access article distributed under the Creative Commons 4.0 Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ©2020 by author. Corresponding Author : Dwi Hilda Putri Department of Biology, Faculty of Mathematics and Natural Science (FMIPA), Universitas Negeri Padang, Indonesia Email : dwihildaputri.08@gmail.com Article history : Received Januray 14, 2020 Revised March 10, 2020 Accepted April 22, 2020 Published April 30, 2020 Article Info Keywords : resistance, endophytic bacteria, extraction, ethanol