Plant Archives Volume 20 No. 2, 2020 pp. 5113-5120 e-ISSN:2581-6063 (online), ISSN:0972-5210 *Author for correspondence : E-mail: surabio84@gmail.com IN VIVO EFFECT OF SPIRULINA PLATENSIS AQUEOUS EXTRACT AND CHITOSAN NANO PARTIALS ON RATS WOUNDS, INDUCED BY PSEUDOMONAS AERUGINOSA Sura H. Nayyef 1 * and Karkaz M. Thalij 2 1* Biology Department, College of Science, Tikrit University, Tikrit, Iraq. 2 Food Science Department, Tikrit University, Tikrit, Iraq. Abstract This study has documented the In Vivo activity for Spirulina platensis aqueous extract (SPAE) and Chitosan nanoparticles (CHNPs) towards Pseudomonas aeruginosa in wound infections of laboratory rats. SPAE at 75% and 200% or Ch-NPs 12.5% each one alone or when combined with Amoxicillin 25 µg were affected significantly on Biological parameter such as Urea, Creatinine, AST, ALT and immune cells that consist (White blood Cells, Lymphocyte, Neutrophil and Immunoglobulin A) of laboratory rats when they induced wounds with P. aeruginosa by decreased these biological parameters compared with infection treatments. The conclusion was showed that SPAE and CH-NPs has antibacterial and biological activity toward the bacterial isolate and biological parameter of rats and this activity increased significantly when mixture with Amoxicillin. Key words : Spirulina platensis, Chitosan nanoparticles, P. aeruginosa, biological effect. Introduction The wound, is defined as disruption in the epithelial lining continuity of the skin, or mucosa resulting from the Physical or thermal damage. The wound is classified as a chronic and acute, according to the nature and duration of healing process (Robson et al., 2001; Dhivya et al., 2015). The pathogenic bacteria are those bacteria that have able to cause disease. Bacterial infection has a major effect on public health (Doron, 2017; Ghimire et al., 2018). Natural products were used as medicines in the rural areas of several developing countries (Hosseinzadeh et al., 2015). Spirulina now called (Arthrospira), produce a wide numbers of the bioactive compounds that has inherent medical advantages (Al-ghanayem, 2017). It is used as food supplements because their rich in nutritional content, with complete protein, lipids and carbohydrates. In addition, Spirulina has antioxidant and antimicrobial properties (Kore and Wakte, 2017). Chitosan is one of the most polymers used in field of Nano drug delivery system (Jesus et al., 2020). (NPs), assumed an important role in the area of drug delivery. Despite the number of studies including NPs are growing over the last years, one of the most studied polymers in the Nano based drug delivery system field is chitosan. NPs of Chitosan have several applications in non- parenteral drugs delivery for treatment of gastrointestinal diseases, pulmonary diseases, gastrointestinal diseases, cancer, brain and ocular infections, in addition to their immune-improving and antimicrobial effect (Mohammed et al.,2017; Divya et al.,2017). Materials and Methods Samples collection 62 wound samples were collected from wound infections of both sexes under sterile conditions by using sterile transport cotton swabs. 9 bacterial isolates were P aeruginosa that used in this experiment. Bacterial isolates Identification Colonies of bacterial isolates were identified, initially with the morphology appearance through notes the color, Consistency and the formed on MaCconkey’s and Nutrient agar medium also according to hemolytic type on blood agar medium. (Alfred, 2005). Microscopic examination Microscopic examination were classified the bacterial