IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.Volume 7, Issue 1, Ver. I. (Feb. 2014), PP 35-40 www.iosrjournals.org www.iosrjournals.org 35 | Page Identification Of Chemistry Bioactivity Structure Of Α- Glucosidase Inhibitor From Ethanol Extract Of The Stem Bark Raru (Vatica Pauciflora Blume) Ida Duma Riris Faculty of Mathematics and Natural Sciences, State University of Medan, Indonesia Abstract: Inhibitory compound of α-glucosidase bioactivity was isolated from Ethanol extract of the stem bark of Raru (Vatica pauciflora Blume) which, is a wild plant that grows in Tapanuli state in Indonesia.The ethanol extract of the stem bark of raru was partitioned and was chromatographed on columns with stationare fase silica gel 60 mesh F 254 (0,063 mm – 0,200 mm). The ethanol extract of isolates showed the enzyme α-glucosidase inhibitor IC 50 of 93.46. The next step is the determination of the chemical structure of the UV Vis, FT-IR, NMR, (COSY, HMQC and HMBC). It found of compounds consist of 2 methoxy, 1 aromatic, and 1 carbonil compounds. The Isolat is a 3,4,9,Trihidroxy-2-(hydroxymethyl)-8-10-dimethoxy-2-3-4atetrahydropyrano(3,2- c)isochromen-6(10bH)-one acording to IUPAC. Keywords: Raru (Vaticapauciflora Blume),antdiabetic, α-glucosidase inhibitor I. Introduction The use of traditional medicine is Indonesian culture that goes back thousands of years ago. It is a cultural heritage of Indonesia that need to be explored, researched, and developed (Hedi, 2007). Consumed plant extracts for the treatment, which is made in the form of decoction, herbal remedies and herbal capsule form. Things like this do to treat diabetes mellitus. Diabetes mellitus (DM) is a condition of the concentration of glucose in the blood is chronically higher than normal value (hyperglycemia). This is caused by a deficiency of insulin or the insulin does not function effectively. Diabetes can lead to various diseases such as hypertension, stroke, coronary heart disease, and kidney failure (Guyton, and Hall, 2007). Measurement of glucose levels can be determined in vitro by enzymatic methods (Lucile, 1997). Spectrophotometer for color intensity is used hereinafter blood glucose levels can be determined. According to WHO (2005), more than 80% of the population in developing countries consume natural ingredients derived mainly from plants, either as a form of maintaining health and medicine. The use of natural medicine is intended to avoid the side effects of chemical drugs. And plant-based treatments currently has a market share of about 30%. Adebayo (2008) found extracts of plants as antidiabetic impact activities such as: Vermoniaamygdalina, Bidenspilosa, Carica papaya, Citrus aurantiifolia, Ocimumgratisimum, Momordica charantia and Morindalucida these plants have been consumed in Nigeria. Likewise Gunawan (2009) discovered a flavonoid in 4 raru plant species, namely (1) CotylelobiummelanoxylumPierre, (2) Shoreabolancarpoides Symington, (3) Cotylelobiumlanceolatumcraib, and (4) Pierre Cotylelobiummelanoxylon be expressed that these compounds can lower blood sugar levels in vitro. There are three compounds that have activity flafonoid as antidiabetic, namely (1) 3-β-hydrokxynaringenin or isoaromadendrin, (2) taxifolin, and (3) 5-hydroxy-3, 4 ', 7 trimethoksiflavonone. Flavonoids extracted with ethanol. Compounds 1 and 2 were found from plant Euphorbia cuneata Vahl, and compounds to the three found in the ethanol extract of Salvia L. verbenaca Flavonoids were identified by spectroscopic data 1 H, 13 C NMR, DEPT, COSY, HMQC and NOESY (Bahar, 2005). Isolation of ethyl acetate extract from the leaves of plants Arto carpus communis flavonoid found to have activity as an antidiabetic, ie 8-geranyl-4,5,7-flavone trihidroksi. This can inhibit α-glucosidase enzyme IC50 18.12 pg mL-1 (Puspa, 2008). In the ethanol extract of the stem bark raru contained flavonoids are phytochemicals. Antidiabetic bioactivity barrier test conducted by Ida Riris Duma (2013) in vitro against hexane, ethyl acetate, ethanol, and water from the plant stem bark raru (Vatica pauciflora Blume), obtained bioactivity of α -glucosidase inhibition of ethanol extract of the stem bark extract is higher than ethyl acetate, hexane and water by using acarbose as a control. In this study bark raru types Vatica pauciflora Blume extracted, and further testing of the inhibitory activity against enzimα-glucosidase. Toxicity tests conducted by Brine Shrimp Lethality Test (BSLT). Further isolation and elucidation performed on extracts that have inhibitory acti vity of α-glucosidase enzyme of the most high. The chemical structure determined by spectroscopic spectral data (UV, FT IR1, NMR1D, 2D NMR (COSY, HMQC, HMBC), and HR MS.