The Validity and Reliability of Science Virtual Test on Levels of Organization and Cell Transport Topic (SVT-LOCT) to Assess Students’ Science Process Skills Wafa Hanifah 1 , Nuryani Rustaman 2 , Lilit Rusyati 3 {wafarkk@gmail.com 1 } International Program on Science Education, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung, 40154, Indonesia 1,3 , Department of Biology Education, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung, 40154, Indonesia 2 Abstract. The purpose of this study was to analyze the validity and reliability of science virtual test to assess junior high school students‟ science process skills on levels of organization and cell transport topics, SVT-LOCT. The final design of the instrument was administered to 118 students of 7th graders from two schools in Kabupaten Bandung, Indonesia. The three steps to validate SVY-LOCT are content analysis of levels of organization and cell transport topics, expert judgment and readability test, and trial test (small group try out and larger test). SVT-LOCT was judged through education, content, media, and language by experts and readability test was conducted by teachers and students. The validation resulted in 31 test items that represent eight skills of SPS to assess students‟ science process skills in levels of organization and cell transport topic. The reliability of SVT-LOCT was considered as high with Cronbach alpha = 0.753 with correlation of XY = 0.65, meaning that the instrument is sufficient to assess students‟ science process skills. Keywords: Science Virtual Test, Science Process Skills, Levels of Organization, Cell Transport 1 Introduction The development of the advance of technology is resulting the competition in the life aspects. The swift improvement of technology has influenced many areas education, including the testing and assessment area. This competition requires some country change their educational testing system that involves technology in the learning process. The computare simulation assessment as an E-test (electronic testing) is one kind of tools that are always used in teaching process to improve the accuracy and efficiency of taking students‟ data [1]. The assessment area where testing method has been alternated from the traditional paper- based test to computer-based test is believed to offer need of technology-based assessment in the past year, especially in Indonesia. Since 2013 the national examination in Indonesia has implemented CBA in the form of computer based-test (CBT). However, in utilizing CBT as assessment tools, there are features in CBT that hasn‟t been utilized well [1]. The CBA has had many advantages, such as decreasing paper use, minimizing cost costs for conducting experiments, providing additional opportunities for testing variants in one trial, and the MSCEIS 2019, October 12, Bandung, Indonesia Copyright © 2020 EAI DOI 10.4108/eai.12-10-2019.2296435