_____________________________________________________________________________________________________ *Corresponding author: E-mail: m.seifzadeh@gmail.com, m_seifzadeh_ld@yahoo.com; Asian Journal of Biology 16(2): 40-49, 2022; Article no.AJOB.61568 ISSN: 2456-7124 The Evaluation and Comparison of Chemical Quality of β-Carotene Extracted in Different Seasons from Azolla filicloides Mina Seifzadeh a* a National Fish Processing Research Center, Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran. Author’s contribution The sole author designed, analysed, interpreted and prepared the manuscript. Article Information DOI: 10.9734/AJOB/2022/v16i2300 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/61568 Received 20 October 2020 Accepted 28 December 2020 Published 17 December 2022 ABSTRACT The present research was aimed for determining the quality of natural β-Carotene, comparing it with synthetic β-Carotene and the effect of season on it. Azolla was sampled in summer and winter seasons. The treatments included β-carotene derived from Azolla through the organic solutions. Synthetic β-Carotene was used as the control. The treatments were kept at 5°C for one year. The results showed purity, concentration, colorimetric and vitamin A in the experimental and control treatments, revealed significant difference (p<0.05). β-Carotene amount was higher in summer treatment group as compared to those sampled in winter (p<0.05). The solubility of β-Carotene was greatest in tetrahydrofuran, while methanol and acetonitrile exhibited the least solubility. Degradation was greatest in cyclohexanone. The experimental treatments had a desirable chemical quality the end of storage period. As the natural β-Carotene takes precedence over the synthetic one in terms of the food hygiene, it is recommended that β-Carotene extracted from Azolla can be substituted with synthetic β-Carotene in the food industry. Keywords: Anzali wetland; Azolla filiculoides; β-Carotene; food hygiene; natural pigment. Original Research Article