SUPPLEMENTARY MATERIAL Anti-inflammatory activity of saponins from the roots of Impatiens parviflora DC. Karolina Grabowska a , Dagmara Wróbel a , Paweł Żmudzki b and Irma Podolak a a Department of Pharmacognosy, Pharmaceutical Faculty, Medical College, Jagiellonian University, Medyczna 9, 30-688 Cracow, Poland b Department of Medicinal Chemistry, Pharmaceutical Faculty, Medical College, Jagiellonian University, Medyczna 9, 30-688 Cracow, Poland Abstract Two triterpene saponins (IPS-1, IPS-2) for the first time were isolated from the roots of Impatiens parviflora DC. (Balsaminaceae). Their anti-inflammatory activity was evaluated by means of two in vitro models: anti-hyaluronidase and anti-denaturation assays. Both saponins were shown to be potent hyaluronidase inhibitors that affect the enzyme in a dose-dependent manner. The antihyaluronidase effect of IPS-2 (IC 50 = 286.7 μg/mL) was higher than that of the reference drug: escin (IC 50 =303.93 μg/mL). Both saponins protected bovine serum albumin from heat-induced denaturation in a dose-dependent manner. IPS-1 demonstrated higher anti-denaturation effect (IC 50 =86.7 μg/ml) than IPS-2 (IC 50 =109.76 μg/mL) or the standard drug: acetylsalicylic acid (IC 50 =262.22 μg/mL). In conclusion, potent activity of IPS-1, IPS-2 in both in vitro assays shows that saponins from I. parviflora have anti- inflammatory activity. The obtained results allow to suggest that such compounds may be beneficial in inflammatory conditions, especially associated with excessive degradation of hyaluronic acid. Keywords: saponins, Impatiens, anti-denaturation activity, hyaluronidase inhibition, anti- inflammatory