Assay methods for SUMO–SIM interactions in vivo and in vitro using a split-luciferase complementation system Mikako Hirohama a,e,g , Arnout R.D. Voet b , Takeaki Ozawa d , Hisato Saitoh e , Yoichi Nakao f , Kam Y.J. Zhang b , Akihiro Ito a,c *, Minoru Yoshida a,c,g a Chemical Genetics Laboratory, b Zhang Initiative Research Unit, RIKEN, c Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan d Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan. e Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. f Department of Chemistry and Biochemistry, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. g Japan Science and Technology Corporation, CREST Research Project, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan * Correspondence should be addressed to Akihiro Ito Tel: +81-48-467-9518 Fax: +81-48-462-4676 E-mail: akihiro-i@riken.jp © 2013. This manuscript version is made available under the Elsevier user license http://www.elsevier.com/open-access/userlicense/1.0/