2017 Vol. 8 No. 5: 65 Research Article 1 © Under License of Creative Commons Attribution 3.0 License | This article is available from: www.acmicrob.com ARCHIVES OF CLINICAL MICROBIOLOGY ISSN 1989-8436 iMedPub Journals www.imedpub.com DOI: 10.4172/1989-8436.100065 Aml El-Sayed Abdou 1 , Ahmed Mora 1 *, Abbas Mohamed Abbas 2 , Mohamed Sayed Hassan 2 , Hala M. Ali 2 and Ahmed Fayed 2 1 Microbiology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt 2 Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt *Corresponding author: Ahmed Mora  ahmedtalaatmora@gmail.com Biochemistry DepartmentAl-Azhar UniversityNaser City 11884, Cairo, Egypt. Tel: +201069893392 Citation: El-Sayed Abdou A, Mora A, Abbas AM, Hassan MS, Ali HM, et al. (2017) Detection of Helicobacter Pylori Infecton in Biliary System in Egyptan Patents with Calcular Obstructve Jaundice. Arch Clin Microbiol. Vol. 8 No. 5:65 Detecton of Helicobacter Pylori Infecton in Biliary System in Egyptan Patents with Calcular Obstructve Jaundice Abstract Background: Bacterial infecton was described as one of the precipitatng factor in cholesterol gallstone formaton and many studies have confrmed the presence of Helicobacter species in the hepatobiliary system. The aim of our study was to detect the presence of Helicobacter species in bile juice by polymerase chain reacton (PCR) in patents presented with calcular obstructve jaundice. Methods: Bile samples from 75 patents presented with calcular obstructve jaundice were obtained during Endoscopic retrograde cholangiopancriatography (ERCP) and subjected to nested PCR for H. pylori DNA detecton and bacterial culture. Gastric biopsies were also taken for H. pylori rapid urease and culture. Results: Helicobacter DNA was detected in 11 out of 75 bile sample by nested PCR, 0 were positve by bile culture, 30 were positve by rapid urease test from gastric biopsy and 5 were positve by gastric biopsy culture. Conclusion: H. pylori was found in the biliary system, in patents with calcular obstructve jaundice suggestng that these bacteria may be of etological importance in gallstone formaton. Keywords: Gallstone disease; Helicobacter; Obstructve jaundice-nested PCR Received: October 08, 2017; Accepted: October 16, 2017; Published: October 23, 2017 Introducton Helicobacter pylori (H. pylori), is a gram-negatve microaerophilic bacterium found usually in the stomach, was frst identfed by Warren and Marshall [1] in 1982 and soon afer it was linked with chronic gastrits and gastric ulcers [2]. Although H. pylori is recognized as a human pathogen associated with gastric lesions, studies have revealed the presence of Helicobacter species with some extragastric diseases such as cardiovascular diseases, lung diseases, hematologic diseases, eye and skin diseases, hepatobiliary diseases, diabetes mellitus, and neurological disorders [3]. Gallstone disease is one of the common problems afectng the digestve tract however, the cause of gallstone formaton beginning with a change in the compositon of bile, leading to stones formaton [4]. Diferent studies suggest that bacterial infecton has an important role in the formaton of brown pigmented gallstones and that the formaton of pure cholesterol gall-stones depends mainly on cholesterol saturaton and solubility [5]. In many studies, the presences of diferent Helicobacter species were shown in the hepatobiliary system [4,6]. Bile-resistant hepatc Helicobacter species such as H. bilis, H. pullorum, and F. rappini were isolated from gall bladder (GB) mucosa and bile juice of patents with chronic cholecystts and gallstones so these agents may be the key elements in the development of various GB-related diseases, especially GB cancer [7]. Diagnosis of H. pylori infecton includes invasive (culture of Biopsy specimens taken by endoscopy) and non-invasive (serologic testng of the patent's serum antbody response to the organism) methods [8,9]. Recently Polymerase chain reacton (PCR) has been a reliable and highly sensitve tool for detecton of H. pylori gene sequences in clinical specimens [10,11].