148 Bulletin UASMV, Veterinary Medicine, 69(1-2)/2012 Print ISSN 1843-5262; Electronic ISSN 1843-5378 Histological Study of the Healing of Bone Defects in Rats Treated with AD-DIEE Type Biophytomodulators Daniela OROS, Liviu OANA, Viorel MICLĂUŞ, Cristian CRECAN, Cosmin PEŞTEAN, Ciprian OBER, Ancu DINCĂ, Lucia BEL USAMV Cluj-Napoca, Romania, Faculty of Veterinary Medicine, orosdaniela@yahoo.com Abstract. Healing of bone lesions lasts longer compared to healing of soft tissues. Bone reconstruction can be finalized as early as 5 to 6 weeks unless disturbing factors intervene. This study aimed to histologically assess the healing of surgically induced bone defects in rats treated with AD-DIEE type biophytomodulators, compared to control group. Adult male Wistar rats were used. Surgeries were performed under general anesthesia and consisted in drilling a bone defect in the femoral shaft, using a dental drill. The animals were divided into 2 groups, control group and test group, treated with AD (DIEE) type biophytomodulators. Bone samples for histological examination were collected at 2, 4 and 6 weeks after surgery. The samples were histologically processed, stained by Masson’s trichrome method and examined under the optical microscope. The findings show that the chosen experimental model corresponds in every aspect, as it provides complete information about the bone defect, its edges, adjacent tissues, femoral shaft and how the periosteum is involved in bone proliferation consolidation. The use of AD (DIEE) type biophytomodulators proved somehow beneficial, stimulating bone proliferation, without any large differences compared to the control group. Key words: rat, bone, healing, biophytomodulator. INTRODUCTION The AD-DIEE Biophytomodulators are devices for energetic loading and balancing, patented by physicist Ancu Dincă. The effect of these devices upon the bone healing phenomena has been previously studied in sheep (Oana et al., 2010, Onisor-Gligor et al., 2010), but the experimental model needed an improvement, due to the impossibility of histologically assessing the perilesional tissues, because the sheep were not euthanized and only the newly formed callus was harvested for further study. Oana et al. (2011) described an experimental model for the histological investigation of bone healing phenomena in rats. The model mimics stable fracture repair, by drilling a partial defect in the femoral shaft of Wistar rats, without interrupting the continuity of the bone. Stable fracture repair means that the fracture ends have been immobilized to give relative clinical stability (not necessarily weight-bearing ability), but have not been rigidly fixed by surgery (McGavin and Zachary, 2007). Oros et al. (2011) have conducted a biochemical study of bone healing in rats treated with AD-DIEE Biophytomodulators. The biochemical tests revealed a higher postoperative increase in the values of osteocalcin and alkaline phosphatase in the animals treated with AD-DIEE type biophytomodulators compared to values obtained in the control group. MATERIAL AND METHODS The biological material used in this study was represented by 24 white Wistar rats, males, 8 months old, clinically healthy, average weight of 250 g. The bone defects were done using a technique