Putra @ Escherichia Coli Bacteria Bioedukasi: Jurnal Biologi dan Pembelajarannya Vol. XIX No. 2 October 2021 96 ESCHERICHIA COLI BACTERIA DETECTION IN DRINKING WATER DEPOTS BUNGO DISTRICT Randi Eka Putra 1) , Titis Wulandari 2) , Tri Wera Agrita 3) STKIP Muhammadiyah Muara Bungo randiekaputra23@gmail.com Water is a very important component in life, such as the use of water as drinking water, all living things, both humans, animals and plants, need water. Part of the human body consists of fluids, so water helps the body to work to carry nutrients from the stomach to all parts of the body. So that a hygienic water source is needed, through a processing process first until the water meets health requirements. The purpose of this study was to detect the presence of Escherichia coli bacteria in drinking water depots in two sub-districts in Bungo Regency, namely (Rimbo Tengah, Pasar Muara Bungo). The test was carried out at the Fish Quarantine Station and Quality Control Laboratory of Class 1 Jambi. Direct sampling with sterile bottles. The sample testing method uses the Most Probable Number (MPN) test with SNI ISO 9308-1:2014. Direct sampling with sterile bottles. The sample testing method uses the Most Probable Number (MPN) test with SNI ISO 9308-1:2014. Keywords: E. coli, Drinking Water Depot, Bungo Regency 1. INTRODUCTION The need for water is very important for life, especially the human body because water is the largest composition of substances that make up the human body, which is 68%. The need for drinking water for each person varies from 2.1 liters to 2.8 liters per day (Irianto 2004). Water is a natural component that is very important for human life. If there is no water, humans cannot live, but water can be dangerous if it is not available with the right conditions, both in quality and quantity (Suryana 2013). According to (Trihono 2010), one of the maintenance of beverages to protect public health is the supervision of the quality of drinking water, this is because drinking water is one of the environmental components that have a large enough role in life. Water sources must go through a processing process first until the water meets health requirements, both in terms of quality and quantity. Quality standards for water media which must follow parameters periodically, namely the need for sanitation hygiene including physical, biological, and chemical parameters. Water contaminated with pathogenic bacteria can endanger health, especially having an impact on the digestive tract which can also cause diarrhea (Anggraeni 2020). According to (Restina, 2019), diarrhea is a disease characterized by changes in the shape and consistency of stool and an increase in the frequency of defecation three or more times a day. The most dominant cause of diarrhea is believed to be due to bacterial contamination in water or Waterborne disease including E. coli, Salmonella and Shigella (Tanto 2014). Waterborne disease is a disease that is transmitted directly through drinking water, where the drinking water contains pathogenic germs, causing the person concerned to become sick. One of the pathogens that cause the disease is E. coli bacteria (Priyono 2011). E. coli bacteria are bacteria that can cause diarrhea. Diarrhea is one of many diseases that can be caused by poor quality of drinking water. Pathogenic microorganisms contained in feces can transmit various diseases when entering the human body, in one gram of feces can contain one billion infective virus particles that can survive for several weeks at temperatures below 10 o C. There are four pathogenic microorganisms contained in feces, namely: viruses, protozoa, worms and bacteria that are commonly found in the type of bacteria E. coli. It is necessary to conduct