JEBAT, 2014, Vol 2, No 2, 42-47 - 42 - Study the Antitumor Activity of Copper (II) Complex of 4-Azomalononitrile Antipyrine on Mice Induced With Earlich Ascites Carcinoma Cells Bahgat A. El-fiky* 1 , Samar A. Aly 2 , Ibrahim H. El-Sayed 3 , Sabah F. El-Abd 3 , Sara S. Abo-Ella 3 1 Department of Animal Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. 2 Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt. 3 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. Corresponding author: Email: bahgat_elfeky@yahoo.com Department of Animal Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. Tel no: 00201004470887. INTRODUCTION Cancer is a class of diseases in which a group of cells display uncontrolled growth, invasion and some- times metastasize. These three malignant properties of cancers differentiate them from benign tumors, which are self-limited, and do not invade or metastasize. Cancer affects people at all ages with the risk for most types increasing with age [1]. The main goal of cancer therapy is to attain the maximum therapeutic damage of tumor cells with the minimum concentration of the drug. This can be achieved, in principle, via selective antitumor preparations, the cytostatic effects of which would be restricted within tumor tissue. While 100% selectivity may be impractical, achievement of reasonably high selectivity seems to be a feasible aim. The bioenergetic status in tumor was selective and affected by the metal complexes. Minimization of signals of high-energy phosphate was observed after injection of the complexes. An increase in the number of DNA single- strand breaks registered in tumor tissue, supporting the suggestion that the complexes may directly affect DNA; however, the action of these complexes as antitumor agents was found to be dependent on the type of tumor cell line tested [2]. Structure and bond properties of copper (II) complexes are of continuous interest in inorganic chemistry and biochemistry [3]. On the other hand, various ligands phosphonate derivatives are of interest because of their broad spectrum of biological properties. Much attention has been focused on synthesis of phosphonate and phosphate esters of N-heterocyclic systems and their platinum (II) and palladium(II) complexes because of their potential [4] and significant antitumor activity [5]. The present work was undertaken to investigate the antitumor activity of new synthesized of copper (II) complex of 4-azomalononitrile antipyrine (L), which was shown to have superoxide dismutase-mimetic activity in vitro, on tumor development. The tumor system used in these studies is Ehrlich ascites carcinoma (EAC) cells implanted Swiss albino mice. MATERIALS AND METHODS Instrumental measurements Elemental analyses (C, H and Cl) were performed by the Microanalytical unit of the Cairo University, Egypt. Metal analysis was estimated using standard method. Infrared (IR) absorption spectra were recorded using KBr discs and a Perkin- HISTORY Received: 24 th of July 2014 Received in revised form: 4 h of October 2014 Accepted: 20 th of December 2014 ABSTRACT Copper (II) complex of 4-azomalononitril antipyrine has been isolated and characterized based on IR spectra, 1 H NMR, elemental analyses, molar conductance, electronic spectra and magnetic moment. A diminished amount of antioxidant enzyme coupled with superoxide production in appears to be general characteristic of the tumor cells. This character can be used in cancer treatment. The present study attempted to investigate the effect of copper (II) complex of 4- azomalononitril antipyrine which has SOD like activity on tumor development using Ehrlich ascites carcinoma (EAC) implemented in Swiss albino female mice. The results showed that copper complex with ligand has anti-cancer activity in all studied parameters with variable ratios. KEYWORDS mice earlich ascites carcinoma cells liver and kidney histopathology anti-oxidant enzymes DNA content JOURNAL ENVIRONMENTAL BIOREMEDIATION & TOXICOLOGY http://journal.hibiscuspublisher.com/index.php/JEBAT