Submit Manuscript | http://medcraveonline.com Introduction In recent years of span in between 2014-2018 Ebola viral disease has spread as epidemic but the emergence of the disease frst occurred in 1976 near the Ebola River, Democratic Republic of Congo. After that the virus has emerged periodically and affected several African countries. Among the reported cases of viral infection around 61% of the cases are fatal by nature. 1 Among the family of viruses Filoviruses is the family under which Ebola virus genera comes that contains the genus Cuevavirus. The six species under the Ebolavirus are well known as follows, Zaire ebolaviruses (Ebola virus (EBOV)), Sudan ebolavirus (Sudan virus), Bundibugyo ebolaviruses (Bundibugyo virus), Tai Forest ebolaviruses (Taï Forest virus), Reston ebolavirus (Reston virus (RESTV)) and the most notorious specie Bombali ebolaviruses (Bombali virus). Major floviruses cause hemorrhagic fever in humans, with high rate of fatality case and Ebola viral disease is a disease which has set of symptoms like severe headache, fever, muscle pain, fatigue along with diarrhea, nausea, abdominal pain and unexpected hemorrhage(Figure 1). 2–4 Figure 1 Graphical abstract Host and disease spread There is some conception about the reserved host source for the spread of Ebola to be African fruit bats which is still a research feld to explore but some evidence reveals that recent, Bombali virus has been found in samples of bats collected in Sierra Leone. Major spread occurs via the use of contaminated needles and syringes causing transmission and amplifcation of the disease. In 1989, there was a true fact revealed the Reston ebolavirus found in research monkeys imported from the Philippines that the virus can transmit through droplets in the but no such viral transmission is possible in humans. During the period of 2014-2015 Ebola outbreaks in West Africa, majority of transmission events were between family members and due to direct contact with the dead bodies who died from viral disease was proved to be one of the most dangerous process. 5–12 Detection techniques Among the detection techniques majorly four types of detection techniques are being focused those are (a) cell culture assay, (b) antibody detection assay, (c) protein antigen detection technique and (d) RT-PCR technique. In cell culture assay techniques to confrm the presence of virus the viral isolation in cell culture, typically using Vero E6 African Green monkey kidney cells are being carried out. The propagated virus is visualized by electron microscopy directly or indirectly visualized by immuno fuorescence microscopy within 1 to 5days of inoculation. Due to the requirement of bio safety level 4 containment, the technique is typically restricted to research and public health laboratories. Next in antibody detection, ELISA is the basis of it. In 1995 out break frst IgM and IgG ELISAs were employed by the CDC during with persistence of IgG and the fnal time point tested was day 117. In recent studies during the 2014-2015 epidemics the onset of IgM and IgG was between 6 and 11days and 9 and 11days after the viral symptom onset respectively. Further the study revealed that the IgM antibody responses are variable, with the onset of detection from 2 to 11days but the IgG responses are detectable in the second week of illness and persist for years which is useful tool for population-level more studies and detection. However, in Protein Antigen Detection, the viral protein antigens circulating in blood provides a dependable J Anal Pharm Res. 2020;9(1):1‒3. 1 ©2020 Bose et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Control and prevention techniques of dreadful “Ebola” virus Volume 9 Issue 1 - 2020 Priyanka Bose, 1 Amit K Harit 2 1 Department of chemistry, Banaras Hindu University, India 2 Department of Chemistry, Korea University, Seoul, Korea Correspondence: Priyanka Bose, Department of chemistry, Banaras Hindu University, Banaras, India, Email Received: September 17, 2019 | Published: January 08, 2020 Abstract Although there is a bunch of disease and disorders are there, Ebola is one of the leading diseases in the world map causing numerous affected beings per year. The viral modifcation with time and wide spreading boundaries are causing more red alters. So the review is focused on the life cycle of the virus, its spread detection technique and new age strategy for drug development to under the control the condition in nutshell. Keywords: ebola, virus, infection, scenario, nutshell, fuorogenic probes, diarrhea, abdominal pain Journal of Analytical & Pharmaceutical Research Review Article Open Access