American Journal of Biochemistry 2016, 6(1): 16-20 DOI: 10.5923/j.ajb.20160601.03 Influence of Catacine on Phospholipase A 2 Activity of Rat Liver in the Dynamics of Chronic Emotional-Painful Stress Development Karim T. Almatov 1,* , Gaffurdzon R. Abdullaev 2 1 Physiology and Biophysics Department, National University of Uzbekistan named after Mirzo Ulugbek, Tashkent, Uzbekistan 2 Human and animal physiology, Namangan State University, Namangan, Uzbekistan Abstract At a chronic emotional-painful stress phospholipase A 2 hydrolytic activity noticeably increases in mitochondria of rats’ liver. Phospholipase A 2 activity increases with increase of experiment’s duration. In our opinion non-bilayer sites may appear in a membrane at a stress and it depends on heaviness of stress. Thusphosphatidylethanolamine is hydrolyzed by phospholipase A 2 quicker in comparison with phosphatidylcholine. Catacine considerably delays influence of negative effect of stress and consequently phospholipase A 2 activity decreases in respect of phosphatidylethanolamine and phosphatidylcholine and comes nearer to normal rates. It means that in stress conditionscatacine increases non-bilayer sites in a cellular membrane of a living cell. Keywords Rat, Chronic emotional-painful stress, Catacine, Liver, Mitochondria, Phosphatidylcholine, phosphatidylethanolamine, Free fatty acids, Phospholipase А 2 1. Introduction At presenta problem of stress and its influence on various functional systems of an organism is still actual problem for modern physiology and medicine [1-6]. Influence of emotiogenic factors breaks the hormonal and vegetative nervous systems homeostasis. In this regard an interest of scientists in stress problem constantly grows and it isn't casual as mechanisms of development of a stress and adaptation of an organism to it are still insufficiently studied, there aren't enough data on the metabolic changes happening in tissues and bodies in stress conditions. A stress is considered as a way of achievement of resistance of an organism to action of extreme factors of various geneses. At the same time, a stress can become the factor having the damaging effect on organs and systems, conducting to development of diseases [7]. The maximum preservation of intellectual and physical powers of human in the conditions of stress is a main problem of modern physiology and medicine. All above-stated facts are also defined the purposes and problems of the present research. Stress phenomenon and adaptation to it are one of the problems facing modern physiology. Despite a large number of the investigations and publications, devoted to various aspects of * Corresponding author: nba_0107@nail.ru (Karim T. Almatov) Published online at http://journal.sapub.org/ajb Copyright © 2016 Scientific & Academic Publishing. All Rights Reserved studying of this problem there are no the exhaustive data containing complete idea of stress as physiological process, mechanisms of its appearance, formation, stress stability in a modern literature; in addition approaches for prophylaxis of negative consequences of stress are not developed. It is known that recently much attention is paid to investigations of various medicines, capable to reduce or prevent the damaging action of stress on tissue membranes and cellular organelles. Under influence of phospholipases, first of all, hydrolysis reactions are progressed, but at the same time synthesis, trans acylation and transalkylation reactions can go. As a result ratio of bilayer and non-bilayer sites in membranes changes considerably. Besides, under influence of phospholipases such physical characteristics of membranes as viscosity, hydrophobicity, and electric parameters can change. Change of structural and physicochemical properties, in turn, has to affect a functional condition of membranes: membrane-bound enzymes activities, permeability for various substances. So, for example, it is shown that functioning of potential-dependent channels essentially depends on a charge of phospholipidmolecules, making a direct environment of the channel [8]. Considering above-stated facts, it is reasonable to assume that it is connected with change of the phospholipid structure of mitochondrial membranes, proceeding with participation of endogenous phospholipases. Therefore, research of a problem of mitochondrial phospholipase participation in the enzymatic