FEMS Microbiology Ecology 86 (1992)303-310
© 1992Federationof European Microbiological Societies 0168-6496/92/$05.00
Publishedby Elsevier
303
FEMSEC 00376
Huge increase in bacterivores on freshly killed barley roots
S. Christensen t, BS. Griffiths 2, F. Ekelund m and R. Ronn t
i Copenhagen University, Department of Population Biology, KObenhavn, Denmark, and z Scottish Crop Research Institute,
Inve~owrie, Scotland, UK
Received14 September1991
Revisionreceived13 November1991
Accepted14 November1991
Key words: Barley root; Bacterivore; Microbial activity; Microfaunal activity
1. SUMMARY
Adding fresh roots to intact soil cores resulted
in marked increases in microbial and microfaunal
activity at the resource islands. Microbial activity
increased in two phases following root addition.
Respiratory activity and concentration of respira-
tory enzyme (dehydrogenase) in soil adhering to
the roots was very high during the first three
weeks resulting in anaerobic conditions in the
soil. After a period of low respiratory activity and
enzyme content, these quantities increased from
6 to 20 weeks, but not enough to maintain anaer-
obic conditions. Numbers of protozoa peaked
earlier than the nematodes. Based on yield coeffi-
cients of microbes and bacterivores, the increase
in bacterivores was in accordance with root-in-
duced respiration activity. In soil adhering to
roots, numbers of bacterial grazers (protozoa and
nematodes) were up to 80 and 30 times higher,
respectively, than in the surrounding soil. This
Correspondence to: S. Christensen, Copenhagen University,
Department of Population Biology, Universitetsparken 15,
DK-2100 KcbenhavnO, Denmark.
effect is up to 20 times higher than observed
around live root systems, which may suggest that
the rhizosphere effect on mierobivores could for
the major part result from the decomposition of
dead segments of the root system.
2. INTRODUCTION
Soil is a highly dynamic medium, where or-
ganic matter inputs to terrestrial ecosystems are
decomposed. Since the resource units are usually
very small (a root fragment, a small soil animal
etc.), steep gradients in decomposition activity
occur over mm distances. A local increase in
available carbon in undisturbed soil following
plant root decay [1] or addition of dead bacterial
cells [2] will lead to the formation of an anaerobic
soil volume, as measured by denitrification under
conditions of no NO~" limitation. To understand
the dynamics of in situ decomposition activity and
population changes requires measurement of soil
respiration geometry and decomposer popula-
tions at the organic resource islands, where the
limiting factors operate. Adding pieces of wood
to forest soil allowed the study of successional
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