1107 Bali Medical Journal 2022; 11(3): 1107-1110 | doi: 10.15562/bmj.v11i3.3467 ORIGINAL ARTICLE ABSTRACT Antioxidant activity of Tinospora crispa extracted with diferent ethanol solvents Renny Novi Puspitasari 1,2* , Sri Agus Sudjarwo 1 , Agung Pranoto 1 , Achmad Basori 1 Introduction: Tinospora crispa (T. crispa) is an herbaceous plant that commonly grows wild in tropical regions of South East Asian countries such as Indonesia, Malaysia, and Thailand. In Indonesia, this plant is well known to be used as a traditional medicine to treat gout, diabetes, hypertension, rheumatic, fever, and appetite stimulant. Researches worldwide indicate that T. crispa poses several pharmacological properties. One of those is the antioxidant activity, acting as a free radical scavenger. The objective of this study was to determine the antioxidative properties of T. crispa and to compare the diferent ethanol solvents used for extraction. Methods: The amount of 300 g T. crispa powder was extracted using 70%, 80%, and 96% ethanol. The spectrophotometry method is used to assess the total favonoid and polyphenol contents as well athe s DPPH assay. Results: The 80% ethanol had the highest favonoid content 0.090% ± [0.453%], while 96% ethanol indicated the lowest 0.038% ± [3.090%]. In the case of phenolic content, 96% ethanol showed the highest result 0.521 ± [11.341%]. However, this value was relatively comparable with the other solvents. The highest DPPH activity was shown by 80% ethanol 6.46 mg ± [3.04 mg]. Conclusion: Despite low in the concentration, favonoid and polyphenol content was successfully determined from T. crispa by using diferent ethanol solvents. Based on the result of antioxidants concentration and activity, 80% ethanol is the most ideal solvent to be used for extraction of T. crispa. Keywords: Tinospora crispa, antioxidant activity, favonoid, phenolic. Cite This Article: Puspitasari, R.N., Sudjarwo, S.A., Pranoto, A., Basori, A. 2022. Antioxidant activity of Tinospora crispa extracted with diferent ethanol solvents. Bali Medical Journal 11(3): 1107-1110. DOI: 10.15562/bmj.v11i3.3467 1 Faculty of Medicine, Universitas Airlangga, Campus A, UNAIR. Jl. Mayjen Prof. Dr. Moestopo, Surabaya, 60132, Surabaya, Indonesia; 2 Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Campus B, UNUSA. Jl. Raya Jemursari, Surabaya, 60237, Surabaya, Indonesia; *Corresponding author: Renny Novi Puspitasari; Faculty of Medicine, Universitas Airlangga, Campus A, UNAIR. Jl. Mayjen Prof. Dr. Moestopo, Surabaya, 60132, Surabaya, Indonesia; dr.renny@unusa.ac.id Received: 2022-06-30 Accepted: 2022-08-11 Published: 2022-09-09 1107 Bali Medical Journal (Bali MedJ) 2022, Volume 11, Number 3: 1107-1110 P-ISSN.2089-1180, E-ISSN: 2302-2914 Open access: www.balimedicaljournal.org INTRODUCTION Te development and progression of several chronic diseases including cancer, neurodegenerative, cardiovascular, diabetes mellitus, and aging have been suggested due to the involvement of oxidative/nitrosative stress. 1,2 Tis condition occurs due to the presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) such as hydroxyl, superoxide, and nitric oxide radicals in the body. 3 Tese molecules can cause not only DNA damage but also oxidation of lipid and proteins in the cell. 4 Antioxidant system in the human body normally has the ability to counterattack these radicals resulting in the balance between oxidation and anti-oxidation. However, the amounts of these radicals are also afected by factors such as lifestyle and environment. Excessive ROS and RNS production can be induced by smoking and alcohol intake as well as radiation and environmental toxins. Hence the balance between oxidation and anti-oxidation was disrupted leading to disease development. 4 Te risk of oxidative stress can be reduced by taking exogenous antioxidants. Medicinal plants are known as valuable sources of exogenous antioxidants. 4,5 Many medicinal plants which have been used worldwide were reported that have an antioxidant activity such as Lamiaceae (rosemary, sage, oregano, marjoram, basil, thyme, mints, balm), Apiaceae (cumin, fennel, caraway), and Zingiberaceae (turmeric, ginger). 5 Antioxidants derived from medicinal plants are mainly polyphenols (phenolic acids, favonoids, anthocyanins, lignans, and stilbenes), carotenoids (xanthophylls and carotenes), and vitamins (vitamin E and C). Tese natural antioxidants in general pose a broad spectrum of biological efects such as antibacterial, antiviral, anticancer, anti- infammatory, and anti-aging. 4 Tinospora crispa (T. crispa) is one of the traditional medicinal plants which has been reported to have antioxidant activity. T. crispa is an herbaceous plant that commonly grows wild in tropical regions of South East Asian countries such as Indonesia, Malaysia, and Tailand. 6 T. crispa is commonly known as Brotowali, Antawali, and Andawali in Indonesia. Te leaves of T. crispa have heart-shaped 6-12 cm long and 7-12 cm wide. Te stems are brownish and feshy with protruded blunt tubercles. 6 Tis plant is well known to be used as a traditional medicine to treat gout, diabetes, hypertension, rheumatic, fever, and appetite stimulant. Te habitat of T. crispa is in the rainforest and deciduous forest with an elevation up to 1000 m. Te suitable soil for growth is moist or dry with sufcient sunlight exposure. 6,7 In this study we cultivated, extracted, and analyzed T. crispa to determine the