RESEARCH PAPER Antimicrobial bionanocomposite–from precursors to the functional material in one simple step Z. Konvičková & A. Schröfel & M. Kolenčík & K. Dědková & P. Peikertová & M. Žídek & J. Seidlerová & G. Kratošová Received: 12 October 2016 /Accepted: 9 November 2016 # Springer Science+Business Media Dordrecht 2016 Abstract The mesoporous biosilica with unique 3D hierarchy in/organic functional groups is attractive material in terms of interfacial phenomena, and its high biocompatibility accelerates development in biomedical devices. In addition, their benefits also play a fundamental role in antimicrobial assessment. We hypothesize that the Diadesmis gallica biosilica surface acts as a biotemplate for AgCl and Au nano- particle (NP) biosynthesis. Moreover, it exhibits antibacterial action human pathogenic bacteria. Nanoparticle biosynthesis was performed via a pure environmental-friendly, static, bottom-up in vitro re- gime. Minimal inhibitory concentrations evaluated systems with bionanocomposites for antibacterial efficiency in temporal time-dose-dependency. TEM and XRD depicts a biosilica Blocal sphere^ which affects formation, stabilization and encapsulation of crystalline Au (9–27 nm) and AgCl (3–51 nm) NPs J Nanopart Res (2016) 18:368 DOI 10.1007/s11051-016-3664-y Z. Konvičková (*) : K. Dědková : P. Peikertová : J. Seidlerová : G. Kratošová Nanotechnology Centre, VŠB – Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic e-mail: konvickova.zuzana@gmail.com K. Dědková e-mail: katerina.dedkova@vsb.cz P. Peikertová e-mail: pavlina.peikertova@vsb.cz J. Seidlerová e-mail: jana.seidlerova@vsb.cz G. Kratošová e-mail: gabriela.kratosova@vsb.cz A. Schröfel Division BIOCEV, Institute of Molecular Genetics of the ASCR, v. v. i, Průmyslová 595, 252 50 Vestec, Czech Republic e-mail: adam.schrofel@gmail.com M. Kolenčík Department of Soil Science and Geology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic e-mail: marekkolencik@gmail.com K. Dědková Regional Materials Science and Technology Centre, VŠB – Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic P. Peikertová IT4 Innovations Centre of Excellence, VŠB – Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic M. Žídek ENET Center, Laboratory of Bulk Materials, VŠB – Technical University of Ostrava, 17.listopadu 15/2172, 708 33 Ostrava, Poruba, Czech Republic e-mail: martin.zidek@vsb.cz