C o p y r i g h t b y Q u i n t e s s e n z Alle Rechte vorbehalten Influence of Hydrostatic Pulpal Pressure on the Microtensile Bond Strength of All-in-one Self-etching Adhesives Keiichi Hosaka a /Masatoshi Nakajima b /Francesca Monticelli c /Marcela Carrilho d / Monica Yamauti e /Juthatip Aksornmuang f /Yoshihiro Nishitani g /Franklin R. Tay h /David H. Pashley i / Junji Tagami j Purpose: To evaluate the microtensile bond strength (μTBS) of two all-in-one self-etching adhesive systems and two self- etching adhesives with and without simulated hydrostatic pulpal pressure (PP). Materials and Methods: Flat coronal dentin surfaces of extracted human molars were prepared. Two all-in-one self-etch- ing adhesive systems, One-Up Bond F (OBF; Tokuyama) and Clearfil S 3 Bond (Tri-S, Kuraray Medical) and two self-etch- ing primer adhesives, Clearfil Protect Bond (PB; Kuraray) and Clearfil SE Bond (SE; Kuraray) were applied to the dentin surfaces according to manufacturers’ instructions under either a pulpal pressure (PP) of zero or 15 cm H 2 O. A hybrid resin composite (Clearfil AP-X, Kuraray) was used for the coronal buildup. Specimens bonded under PP were stored in water at 37°C under 15 cm H 2 O for 24 h. Specimens not bonded under PP were stored under a PP of zero. After stor- age, the bonded specimens were sectioned into slabs that were trimmed to hourglass-shaped specimens, and were sub- jected to microtensile bond testing (μTBS). The bond strength data were statistically analyzed using two-way ANOVA and the Holm-Sidak method for multiple comparison tests (α = 0.05). The surface area percentage of different failure modes for each material was also statistically analyzed with three one-way ANOVAs and Tukey’s multiple comparison tests. Results: The μTBS of OBF and Tri-S fell significantly under PP. However, in the PB and SE bonded specimens under PP, there were no significant differences compared with the control groups without PP. Conclusions: The μTBS of the two all-in-one adhesive systems decreased when PP was applied. However, the μTBS of both self-etching primer adhesives did not decrease under PP. Keywords: dentin, pulpal pressure, all-in-one adhesives, microtensile bond strength. J Adhes Dent 2007; 9: 437-442. Submitted for publication: 12.09.06; accepted for publication: 20.11.06. Vol 9, No 5, 2007 437 a Research Associate, Cariology and Operative Dentistry, Department of Restora- tive Sciences, Tokyo Medical and Dental University, Tokyo, Japan. b Lecturer, Cariology and Operative Dentistry, Department of Restorative Sciences, Tokyo Medical and Dental University, Tokyo, Japan. c Postdoctoral Researcher, Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain; Department of Restorative Dentistry and Dental Materials, University of Siena, Policlinico Le Scotte, Siena, Italy. d Postdoctoral Research Assistant, Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, Brazil. e Assistant Professor, Department of Cariology and Operative Dentistry, School of Dentistry, FEAD, Belo Horizonte, Brazil. f Lecturer, Department of Prosthetic Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand. g Lecturer, Department of Operative Dentistry, Study of Biofunctional Recovery and Reconstruction, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. h Associate Professor, Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta, Georgia, USA. i Regents’ Professor, Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia, USA. j Dean and Professor, Cariology and Operative Dentistry, Department of Restora- tive Sciences; Center of Excellence Program for Frontier Research on Molecu- lar Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Den- tal University, Tokyo, Japan. Reprint requests: Keiichi Hosaka, Cariology and Operative Dentistry, Depart- ment of Restorative Sciences, Tokyo Medical and Dental University, 1-5-45 Yushi- ma, Bunkyo-ku, Tokyo 113-8549, Japan. Tel: +81-3-5803-5483, Fax: +81-3-5803- 0195. e-mail: hosaka.ope@tmd.ac.jp brought to you by CORE View metadata, citation and similar papers at core.ac.uk provided by Repositorio da Producao Cientifica e Intelectual da Unicamp