J Food Process Preserv. 2019;00:e14265. wileyonlinelibrary.com/journal/jfpp
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https://doi.org/10.1111/jfpp.14265
© 2019 Wiley Periodicals, Inc.
1 | INTRODUCTION
Edible films can be defined loosely as an edible thin and continuous
layer that can be formed as food coatings and placed between food
components with the ability to act as a mass transfer barrier, food in‐
gredient/additive carrier, and improving the mechanical integrity or
handling characteristics (Krochta, 1992). Research and development
of environmentally friendly edible packaging is of high interest due to
food processors’ needs for novel storage techniques and new mar‐
ket outlets (Gennadios & Sumner, 1999). In addition, the low time to
store food due to microbial contamination and the use of artificial
carcinogenic preservatives have encouraged various studies in food
packaging, especially edible film technology involving natural preser‐
vatives sourced from animals, plants, and microorganisms. Foods con‐
taminated with microbes will experience changes in texture and taste.
Therefore, they are not suitable for consumption (Gram et al., 2002).
Received: 28 May 2019
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Revised: 17 September 2019
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Accepted: 9 October 2019
DOI: 10.1111/jfpp.14265
ORIGINAL ARTICLE
Physiochemical and antibacterial analysis of gelatin–chitosan
edible film with the addition of nitrite and garlic essential oil by
response surface methodology
Faridah Handayasari
1
| Nugraha Edhi Suyatma
1,2
| Siti Nurjanah
1,2
1
Department of Food Technology, IPB
University, Bogor, Indonesia
2
Southeast Asian Food and Agricultural
Science and Technology (SEAFAST)
Center, IPB University, Bogor, Indonesia
Correspondence
Nugraha Edhi Suyatma, Department of
Food Technology, IPB University, Kampus
IPB Darmaga, Bogor 16680, Jawa Barat,
Indonesia.
Email: nugrahaedhi@yahoo.com
Abstract
The mechanical properties of edible films upon variation of gelatin (G) and chitosan
(C) concentration (G%/C%) were compared using constrained mixture and response
surface methodology (RSM) and their antibacterial activities against P. aeruginosa, B.
cereus, and L. monocytogenes by the addition of sodium nitrite and garlic essential oil
(GEO) were analyzed. Both methods show that increasing chitosan concentration will
gradually enhance edible film tensile strength but decrease their elongation at break.
Optimization to find the gelatin–chitosan edible film with the maximum elasticity was
performed by minimizing the Young’s modulus which yields a value of 0.41 MPa cor ‐
responding to a G60/C40 gelatin–chitosan concentration using constrained mixture
and 0.31 MPa corresponding to a G76/C24 gelatin–chitosan concentration using the
RSM method. Antibacterial analysis of the edible film showed that the addition of
sodium nitrite and GEO in gelatin–chitosan edible film enhances their antibacterial
activity significantly and increasing the GEO concentration also improve the antibac‐
terial activity of the film.
Practical applications
The outcome of our work has practical applications in the field of biodegradable ed‐
ible film, for example, for environmentally friendly meat coatings due to its optimum
elasticity property. In addition, our gelatin–chitosan film with garlic essential oil also
exhibits significant antibacterial properties which can compete with commercial film
that uses nitrite addition and therefore prolong the shelf life of the food without
the risk of carcinogenic effects. From the potential point of view, our work should
stimulate more research toward the development and optimization of biodegradable
edible films with noncarcinogenic antibacterial properties.