Proceedings of the International Colloqium on Environmental Education, Istanbul, September 25-26, 2021 © ICEE 2021 The Effectiveness Test Of Wound Healing Daun Gatal (Laportea Decumana) Against Mice (Mus Musculus L) Ratna Prabawati, Waskito Aji Suryo Putro, Yusnita La Goa, Lukman Hardia, Dyah Putri Utami Universitas Pendidikan Muhammadiyah Sorong, Universitas Diponegoro ratnaprabawati58@gmail.com, waskito_asp@ymail.com yusnitalagoa81@gmail.com lkman.hardia@gmail.com, dyah.utami@arjunawijaya.com Abstract Daun Gatal (Laportea spp) is one of the shrubs that are widely distributed in Papua from the coast to the mountains. Daun Gatal (Laportea spp) has been used for generations by the Papuan people as painkillers. Daun Gatal (Laportea spp) contains compounds monoxide, tryptophan, histidine, alkaloids, flavonoids, formic acid, and anthraquinones. This content is called "antacid" because it gives a sensation like being bitten by an ant. There are many itchy leaves in the village but often they are just left to dry, wither, die, and even be thrown away. The value of this leaf is very large if it is developed not only as an itchy leaf sheet but as a pharmaceutical product. (Simaremare, et al, 2019). This is supported by several research results stating that itchy leaf extract contains compounds of the alkaloid group, glycosides, steroids (Simaremare, 2014), also contains triterpenoid compounds and formic acid (Chrystomo, et al., 2016) and (Krisna and Santanina, 2019) which states that itchy leaves provide antibacterial activity. The type of research used is experimental research. The research design used was a randomized control group pretest and posttest design. The population in this study was white mice. The samples used in this study were white mice that had met the following inclusion and exclusion criteria. Inclusion criteria were female mice, bodyweight 20-30grams, age 2-4 months while the exclusion criteria. Included in the exclusion criteria in this study were mice that were sick or died in the study conditions. The results of the Extraction of Daun Gatal (Laportea Decumana), namely the itchy leaf sample that has been weighed at a concentration of 25% obtained from a mixture of itching leaf extract (25 grams) added with water (30 ml) produces 18.5 ml. Itchy leaf extract at a concentration of 50 % was obtained from a mixture of itchy leaf extract (50 grams) added with water (30 ml) to produce 20.1 ml. Gatak leaf extract at a concentration of 75% was obtained from a mixture of itching leaf extract (75 grams) added with water (30 ml) to produce 24.3 ml. The extraction method used is extracting the extract of itchy leaves. This method was chosen because the process is simple and does not involve heating so that it can prevent damage to chemical compounds that are not resistant to heating, especially flavonoids contained in itchy leaves. Based on the results of the data on the difference in wound diameter of mice, it showed that Treatment Group 1 with 25% itching leaf extract and Treatment Group 2 with 50% itching leaf extract almost had the same healing rate. Meanwhile, Treatment Group 3 with 75% itching leaf extract had the fastest healing rate among other concentrations. In contrast to Treatment Group 3, the control group had a much longer healing rate among other concentrations. Keywords: Daun Gatal (Laportea spp), Extraction, Healing 1. Introduction Human civilization has long used plants in its life. The use of plants as a medium for overcoming disease or maintaining health in the lives of Indonesian people is still ongoing. The types of plants used by each community group (ethnic/ethnic) are numerous and can be different in each group and this knowledge is still passed on to the next generation (Puro, 2012). Daun Gatal (Laportea spp.) is one of the shrubs that are widely distributed in Papua from the coast to the mountains. Daun Gatal (Laportea spp.) has been used for generations by the Papuan people as painkillers. Daun Gatal (Laportea spp.) contains compounds monoxide, tryptophan, histidine, alkaloids, flavonoids, formic acid, and anthraquinones. This content is called "antacid" because it gives a sensation like being bitten by an ant. There are many itchy leaves in the village but often they are just left to dry, wither, die, and even be thrown away. The value of this leaf is very large if it is developed not only as an itchy leaf sheet but as a pharmaceutical product. (Simaremare, et al, 2019). This is supported by several research results stating that itchy leaf extract contains compounds of the alkaloid group, glycosides, steroids (Simaremare, 2014), also contains triterpenoid compounds and formic acid (Chrystomo, et al., 2016) and (Krisna and Santanina, 2019) which states that itchy leaves provide antibacterial activity. Scattered itching leaves have been widely used by the people of India and the people of Papua as food and as medicinal ingredients, including for wound healing, reducing fever, and relieving pain (Lense, 2012). With the content and