How to Cite: Mohammed, M. M. O., Abdul-kader, H. A., & Esho, K. B. (2022). Induction of mutation by treatment with colchicine and spraying with progesterone for two cultivars of zinnia elegans by using DNA molecular. International Journal of Health Sciences, 6(S9), 2927–2951. https://doi.org/10.53730/ijhs.v6nS9.13159 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 9 May 2022, Manuscript revised: 18 July 2022, Accepted for publication: 27 August 2022 2927 Induction of mutation by treatment with colchicine and spraying with progesterone for two cultivars of zinnia elegans by using DNA molecular Maab M. O. Mohammed University of Mosul, College of Agriculture and forestry, Department of horticulture and landscape design Hala Abd. Abdul-kader University of Mosul, College of Agriculture and forestry, Department of horticulture and landscape design Email: Hala62_Iraq@uomosul.edu.iq Kamal B. Esho University of Mosul, College of Agriculture and forestry, Department of horticulture and landscape design Email: kamalesho@uomosul.edu.iq or kamalesho@rocketmail.com Abstract---The study was carried out in the fields of the Department of Horticulture and Landscaping, College of Agriculture and Forestry, University of Mosul, during the agricultural season spring 2021, to study the development of the mutation by treatment with colchicine and spraying with progesterone for two cultivars of zinnia, where two cultivars of zinnia (red and white), and three concentrations of colchicine at 0, 0.05 and 0.1%, and three concentrations of progesterone, which are 0.5, 10 mg / liter. And the study of genetic markers using RAPD technology, where four primers were used. The results obtained are summered up in the occurrence of chromosomal duplication for the two cultivars, and the stomata density was affected as a result of the interaction between cultivars and colchicine, the red cultivar was superior with 0.1% colchicine significantly. And progesterone at 5 mg/L had the highest value in the stomatal area, and the triple interaction treatment between the red variety and 0.1% colchicine and 5 mg/L of progesterone produced the highest value in the stomatal density, and the highest stomatal area resulted when the triple interaction between the white variety and 0% colchicine And 5 mg/L of progesterone. The initiators also showed different bundles in the number and locations, as the primer OPB-10 showed a unique