Research Article Synthesis and Bactericidal Properties of Hyaluronic Acid Doped with Metal Nanoparticles Galo Cárdenas-Triviño, 1 Macarena Ruiz-Parra, 2 Luis Vergara-González, 3 Javier Ojeda-Oyarzún, 4 and Guillermo Solorzano 5 1 Facultad de Ingenier´ ıa, Centro de Biomateriales y Nanotecnolog´ ıa, Universidad del B´ ıo-B´ ıo, Av. Collao 1202, Concepci´ on, Chile 2 Facultad de Ciencia, Qu´ ımica y Farmacia, Universidad San Sebasti´ an, Lientur 1457, Concepci´ on, Chile 3 Facultad de Ciencia, Departamento de Ciencias Biol´ ogicas y Qu´ ımicas, Universidad San Sebasti´ an, Lientur 1457, Concepci´ on, Chile 4 Facultad de Medicina Veterinaria, Universidad Austral de Chile, Independencia 641, Valdivia, Chile 5 Departamento de Engenharia Qu´ ımica e de Materiais, Pontifcal Catholic University of Rio de Janeiro, R. Marquˆ es de S˜ ao Vicente 225, G´ avea, Rio de Janeiro, RJ, Brazil Correspondence should be addressed to Galo C´ ardenas-Trivi˜ no; gcardenas@ubiobio.cl Received 23 March 2017; Accepted 9 October 2017; Published 17 December 2017 Academic Editor: Raul Fangueiro Copyright © 2017 Galo C´ ardenas-Trivi˜ no et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A study on the nanoparticles size and the antibacterial properties of hyaluronic acid (HA) doped with nanoparticles is reported. Nanoparticles from gold, silver, copper, and silver palladium with HA support were performed. Te solvated metal atom dispersion (SMAD) method with 2-propanol and HA was used. High-resolution transmission electron microscopy (HRTEM), infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA) were conducted. Te average sizes of nanoclusters were as follows: HA-Au = 17.88nm; HA-Ag = 50.41nm; HA-Cu = 13.33nm; and HA-AgPd = 33.22nm. Te antibacterial activity of solutions and flms containing nanoparticles against American Type Culture Collection (ATCC) bacterial strains Escherichia coli (EC), Staphylococcus aureus (SA), Staphylococcus epidermidis (SE), and Pseudomonas aeruginosa (PA) was determined. Inhibition was observed for HA-Ag, HA-Cu, and HA-AgPd. Toxicological tests were performed in rats that were injected intraperitoneally with two concentrations of gold, copper, silver, and silver-palladium nanoparticles. No alterations in hepatic parameters, including ALT (alanine aminotransferase), GGT (gamma-glutamyl transpeptidase) bilirubin, and albumin, were observed afer 14 days. Tese flms could be used as promoters of skin recovery and Grades I and II cutaneous burns and as scafolds. 1. Introduction Nanotechnology is now one of the highest priorities in science and technology research [1]. By controlling the size, shape, and distribution of nanoparticles (NPs), it is possible to determine their properties and the nature and intensity of their interactions with other molecules. One way to modify the fnal properties of a nanomaterial is to use a poly- meric support. Te unique properties of supported metallic nanoparticles (MNPs) are directly related to the types of particles (size and shape), the dispersion, concentration, and electronic properties of the metal in the support [2, 3]. MNPs dispersed in solvents have been widely studied over the past few years because their properties have enabled applications in the areas of electronics, medicine, environmental science, and materials science [4]. Nanomedicine is a feld that encompasses applied nan- otechnology methods in medicine. Tus, it allows the use of nanotechnology in cutting-edge research that will lead to the creation of better techniques for the treatment of diseases to improve the prognosis of patients [5]. Te revolutionary aim of this science is focused primarily on monitoring, repairing tissue, controlling the evolution of disease, and defending and improving human biological systems. Te most signifcant biotechnological advances in this feld include biosensors, novel pharmacoselective agents, and the development of materials for grafs, among others [6]. Hindawi Journal of Nanomaterials Volume 2017, Article ID 9573869, 9 pages https://doi.org/10.1155/2017/9573869