Spatial periodicity induced by a chemical wave train Shrabani Sen, Pushpita Ghosh, Syed Shahed Riaz, * and Deb Shankar Ray Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India Received 27 October 2009; published 14 January 2010 An all-chemical analog of clock-wave-front model for somitogenesis is proposed. The spatial periodicity can be obtained by arresting the homogeneous oscillations in a typical two-component reaction-diffusion system in the Hopf region by interacting with a chemical wave front. The patterns can be controlled by tuning the wave speed of the front. DOI: 10.1103/PhysRevE.81.017101 PACS numbers: 82.40.Ck, 89.75.Kd, 47.54.-r The theory of morphogenesis 1proposed by Turing in the middle of the last century is based on the search for symmetry breaking of a homogeneous steady state of a dy- namical system governed by the reaction-diffusion equa- tions. The underlying instability owes its origin to short- range activation and long-range diffusion of two interacting species obeying activator-inhibitor reaction kinetics and the resulting symmetry-broken state is characterized by various spatial regions with distinct physical and chemical features or conspicuous patterns. The theory and its variants have found extensive applications in several areas of natural sci- ences, particularly, in developmental biology 211, where the growth of sequential space-periodic structures along the anterio-posterior axis of the vertebrate embryo has remained a focus of major attraction. Somitogenesis 315provides a typical example of how such spatial periodicity forms during embryogenesis. Several models 814of somitogenesis are now known. Among them the clock and wave front model 14has gained a good testing ground for further experiment. The model assumes the existence of a biochemical oscillator within the band of paraxial mesodermal cells. The oscillator of all the neighboring cells oscillates synchronously. The model further postulates that a wave front of cell change sweeps posteriorly through the cells slowly, halting the os- cillations and thereby inducing maturation of somatites which are essentially blocks of cells of paraxial mesoderm arranged as spatially periodic structures. In the present Brief Report we look for a chemical analog of the clock-wave-front model of somitogenesis. Chlorine dioxide-iodine-malonic acid CDIMAsystem 1621, which has remained as a classic paradigm for a wide class of far-from-equilibrium phenomena in spatially extended sys- tem, offers itself as an excellent candidate for the model. With CDIMA systems it has been shown that progressive arrest of homogeneous oscillations can control the symmetry as well as the wavelength of spatial structures when the re- action medium is illuminated with a constant or periodic source of light 22,23. Our endeavor in this Brief Report is to suggest an all-chemical analog for exploring the possibil- ity of formation stationary pattern out of homogeneous os- cillations in the Hopf region by interaction with a progres- sive chemical wave front or pulse rather than light field. We exploit the sensitivity of CDIMA system to the concentration of some external reducing agent to demonstrate how the wavelength of the produced patterns changes with the veloc- ity of the traveling wave and frequency of the temporal os- cillations. To begin with we consider the following chemical reac- tions that are involved in the CDIMA model 16,17; MA +I 2 I MA +I - +H + , ClO 2 +I - ClO 2 - + 1 2 I 2 , ClO 2 - + 4I - + 4H + Cl - + 2I 2 + 2H 2 O. When starch is added from outside in the reaction medium, we have the additional equilibrium between starch and io- dide ions; S +I 3 - SI 3 - . Ideally the reaction schemes imply a many variable model for CDIMA system. However in the course of reaction the concentrations of all the species but I - and ClO 2 - remain more or less constant. This effectively reduces the model into a two-variable one with concentrations of I - and ClO 2 - play- ing the role of activator and inhibitor respectively. With I -  u and ClO 2 -  v and treating concentrations of all other species as constants Lengeyl and Epstein have sug- gested the governing reaction-diffusion dynamics of these two variables as follows: u = a - u -4 uv 1+ u 2 + 2 u , 1 v = bu - uv 1+ u 2 + d 2 v . 2 Here the parameters a and b are proportional to the con- centration ratios MA/ I 2 and ClO 2 / I 2 , respectively, and are related to kinetic parameters. d refers to the ratio of the diffusion coefficients d = D ClO 2 -/ D I -. is the concen- tration of starch which forms a complex with I 3 - such that =1+ KS, where K is the equilibrium constant for the com- plexation reaction and Sis the concentration of starch. The complexation thus separates the time scales for the * Also at Dept. of Chemistry, Belur Ramakrishna Mission Vidya- mandira, Howrah, 711202, India. pcdsr@iacs.res.in PHYSICAL REVIEW E 81, 017101 2010 1539-3755/2010/811/0171014©2010 The American Physical Society 017101-1