AAB Bioflux, 2019, Volume 11, Issue 3. http://www.aab.bioflux.com.ro 132 Effect of different storage environment on sorghum ( Sorghum bicolor (L.) Moench.) seed vigor Ramlah Arief, Fauziah Koes, Oom Komalasari Indonesian Cereal Research Institute, Jl. Dr.Ratulangi 274 Maros, South Sulawesi, Indonesia. Corresponding author: R. Arief, ramlah.arief@yahoo.com Abstract . The research to evaluate the effect of different storage environment to sorghum ( Sorghum bicolor ) seed vigor was conducted in seed laboratory of Indonesian Cereal Research Institute. The treatments were arranged in completely randomized design with 2 sorghum varieties (Numbu and Super 1) in two storage environment: warehouse storage and cool storage. Observation on seed moisture content, germination percentage, germination rate, shoot and root length, shoot and root dry weight, electric conductivity of seed leakage were carried out in every storage period (0, 4, 8, 12 months). At 12 month storage, Numbu variety showed higher increase of moisture content (34.44%) than that of Super 1 variety (24.18%). Germination percentage has still remained at 99-100% with germination rate of 31.1-32.2%/day until 4 months storage but signficantly decreased at 3% (8 months) and 9% (12 months) on Super 1 variety, decreased on Numbu variety to 4% (8 months) and 12% (12 months) in cool storage. In warehouse storage, at 4, 8 and 12 months storage, germination percentage significantly decreased to 9%, 19% and 25% respectively on Super 1 variety, while for Numbu variety the decrease was 10%, 24%, and 30% respectively. Electrolyte leakage has significantly increased with decrease of germination, germination rate, root length, shoot length, root dry weight, shoot dry weight in every period storage. Storing the sorghum seeds in cool storage showed lower decrease of its germination, germination rate, root length, shoot length and lower electrolyte leakage than in warehouse storage. Key Words: storage, seed quality, sorghum, aging, vigor. Introduction . Sorghum ( Sorghum bicolor (L.) Moench.) grain in Indonesia was used as food in East Nusa Tenggara, South Sulawesi, Central Sulawesi, East and Central Java, and many other areas (ICERI 2018). In East Flores District of East Nusa Tenggara Province, sorghum grain was used as principle staple food. In many sorghum producing areas, productivity is still low, on average of 500-750 kg ha -1 . Poor seed quality is one of the most important factors contributing to this low productivity. Sorghum seed industry is still not well developed in Indonesia. Farmers usually keep part of their produce to be used as seed in the following planting season, or in many cases, farmers may obtain seeds from the local market or from other farmers. They usually store sorghum seed in traditional storage facilities and different type of warehouses. Seeds were usually stored for varying lengths of time after harvest. Seed viability at the end of any storage period is the result of the initial viability at harvest, initial moisture content before storage, temperature and humidity of storage and length of seed storage (Arief et al 2013). Factors affecting seed quality in the storage are environmental condition during seed production, seed viability at harvest, initial seed moisture content, mechanical damage during seed processing, packaging material, temperature and humidity in storage and biochemical injury of seed tissue (TeKrony et al 1987; Anfinrud 1997; Al- Yahya 2001). According to Copeland & McDonald (2001), for one year seed storage seed moisture content should be less than 11% and the temperature should not exceed 20 o C. Seed storage longevity is influenced by seed quality and storage conditions. Low initial seed quality, unfavourable storage condition (storage temperature and relative humidity), contribute to accelerating seed deterioration in storage. The effective storage period is difficult to asses because the storability of the seed is a function of initial seed quality and the storage conditions (Anfinrud 1997; Fabrizius et al 1999). Some tests such