resistance, in patients with type 1 diabetes (T1D). Over the past decades, the profile of T1D patients has considerably evolved. In contrast to the historical view of patients with T1D as being lean, it now appears that up to 45% of them also have the metabolic syndrome (MS) (clinical surrogate of DD). DD appears to have major detrimental health consequences as it has been associated to poor glycemic control, increased risk of micro- and macrovascular complications and even mortality. T1D patients seem to be particularly at risk for lifestyle factors that promote weight gain and its associated MS components: hypoglycemia management (may lead to an excessive caloric intake), adher- ence to an atherogenic diet (bypass of carbohydrates counting difficulties) and a low level of physical activity (PA) (hypogly- cemia fear). These unhealthy lifestyle habits may contribute to the emergence of DD. Objective: Compare energy expenditure related to PA and food habits in terms of quality and quantity of patients with and without DD. Methods: A cross-sectional study among 150 T1D patients with or without DD is currently being conducted to analyse DD compo- nents, dietary profile (3-day food record) and energy expenditure related to PA (SenseWear Armband). Food quality will be assessed using the Canadian Healthy Eating Index score and food quantity using Food Processor software. Preliminary results: Data collected from 118 patients confirm a prevalence of 37% of DD and its association with microvascular complications. Impact: Identifying modifiable risk factors for DD will guide health professionals in their approach with T1D patients. 16 Impact of Cod Protein on Insulin Sensitivity in Women With Polycystic Ovary Syndrome E. TALBOT 1, 2 , S.J. WEISNAGEL 3 , J. MAROIS 1, 2 , H. JACQUES 1, 2 1 Institut des nutraceutiques et des aliments fonctionnels 2 Département de sciences des aliments et de nutrition, Université Laval 3 Unité de recherche sur le diabète, Centre de recherche du CHUL Objective: To compare the effects of cod protein (CP) with those of other animal proteins on insulin sensitivity and lipid profile in women with polycystic ovary syndrome (PCOS). Methods: Nine insulin-resistant women with PCOS were fed a CP diet and a similar diet containing beef, pork, veal, eggs, milk and milk products (BPVEM) for 3 months each, within a crossover design. CP and BPVEM were given in one meal per day. Before and after each experimental period, serum lipids were measured, glucose metabolic response was determined using an oral glucose tolerance test (OGTT) and insulin sensitivity to a hyperinsulinemic- euglycemic clamp. Results: During the OGTT, the serum glucose response was lower 15 minutes (P ¼ 0.046) and tended to be lower 30 minutes (P ¼ 0.07) after glucose challenge in women fed CP than in those fed BPVEM. Serum insulin was decreased at 15 minutes (P ¼ 0.04) and 30 minutes (P ¼ 0.02) and tended to be lower at 60 minutes (P ¼ 0.06) with CP compared with BPVEM. C-peptide was decreased at 15 minutes (P ¼ 0.02) and tended to decrease at 30 minutes (P ¼ 0.05) when women were fed CP compared with BPVEM. The IAUC for serum glucose (P ¼ 0.04), insulin (P ¼ 0.03) and C-peptide (P ¼ 0.03) calculated up to 30 min was significantly lower after the CP diet compared to the BPVEM diet. No significant difference was observed on insulin sensitivity (M/I) and lipid profile. Conclusion: These results suggest that a CP meal per day may reduce the glycemic and insulinemic responses during the early phase of the OGTT in women with PCOS. 17 Physical Activity Practice in the Years Following Delivery and Glucose Homeostasis in Women With Prior Gestational Diabetes JESSICA VIGNEAULT 1, 2 , VÉRONIQUE GINGRAS 1, 2 , ANDRÉ TCHERNOF 1, 2, 3 , S. JOHN WEISNAGEL 3, 4 , JULIE ROBITAILLE 1, 2 1 Department of Food Science and Nutrition, Laval University 2 Institute of Nutraceuticals and Functional Foods, Laval University 3 Endocrinology and Nephrology, Laval University Medical Research Center 4 Diabetes Research Unit, Laval University Medical Research Center, Qc, Canada Our objective was to examine the association between physical activity practice in the years following delivery and glucose homeostasis in women with prior gestational diabetes (GDM). The analysis included 96 women who had GDM between 2003 and 2010. Physical activity practice was measured with accelerometers and time spent weekly in moderate to vigorous physical activity (MVPA) was derived. Plasma glucose and insulin were obtained from a 2-hour 75 g oral glucose tolerance test (OGTT). The Matsuda index for insulin sensitivity was calculated. Women were 36.34.9 years old with a BMI of 27.26.4 kg/m 2 . Testing occurred 2.92.2 years following delivery. 57.3% of women were overweight or obese (BMI 25 kg/m 2 ) and only 31.3% of women accumulated at least 150 minutes of MVPA per week. A total of 22.5% of women were diagnosed with impaired fasting glucose (IFG), 45.0% with impaired glucose tolerance (IGT) and 16.7% with type 2 diabetes. MVPA was negatively correlated with 2h-post OGTT insulin and glucose (r¼-0.30 and r¼-0.23, p<0.05 for both) and was positively corre- lated with the Matsuda index (r¼0.22, p¼0.04). MVPA was a significant predictor of IFG and IGT (r 2 ¼0.05 and r 2 ¼0.07, p0.05), independently of age, BMI and parity. These analyses suggest that, in the years following delivery, regular MVPA play an important role in glucose homeostasis for women with prior GDM. 18 Monoacylglycerol as a Metabolic Coupling Factor in Glucose- Stimulated Insulin Secretion SHANGANG ZHAO, JOSE IGLESIAS, YVES MUGABO, MARIE-LINE PEYOT, ERIK JOLY, S.R. MURTHY MADIRAJU, MARC PRENTKI Montreal Diabetes Research Center, CR-CHUM Glycerolipid/free fatty acid cycle is an essential component of the lipid amplification pathway of glucose stimulated insulin secretion (GSIS) by b-cells. However, little is known about the lipid metabolic coupling factor(s) involved in this process. We now provide evidence that monoacylglycerol (MAG) acts as a metabolic coupling factor in GSIS. In rat islets and INS832/13 b-cells MAG levels increase in the presence of high glucose concentration. Inhibition of the major membrane-bound MAG hydrolase, ABHD6, in INS832/13 cells and islets with WWL70 leads to accumulation of MAG with concomitant increase in GSIS. The predominant MAG species elevated are the saturated long chain MAGs 1-stearoylglycerol (C18:0) and 1-palmi- toylglycerol (C16:0), which strongly potentiate GSIS in vitro, unlike monounsaturated and polyunsaturated MAGs. Overexpression and RNAi-knockdown of ABHD6 in INS832/13 cells, resulted in decreased and increased GSIS, respectively. Administration of WWL70 (i.p.) in normal CD-1 mice enhance GSIS, and it greatly improves glucose tolerance by increasing insulin secretion in the low-dose strepto- zotocin type 2 diabetes mouse model. We also show that MAGs can bind the C1-domain of the exocytotic effector protein, Munc13-1 more efficiently than diacylglycerol, and as efficiently as the phorbol ester PMA, an established activator of Munc13-1. Collectively, the results provide strong evidence that the lipid amplification arm of GSIS in b-cells is mediated by MAGs that activate insulin exocytosis via Munc 13-1. ABHD6 is the major MAG hydrolase in the ß-cell and is a new target for the development of anti-diabetic drugs. Abstracts / Can J Diabetes 37 (2013) 58e62 62