Jayasri et al. 2017/ J. Livestock Sci. 8: 172-175 172 Studies on nephrotoxicity due to combined effect of Aflatoxin and Ochratoxin in broilers and their amelioration with adsorbents A. Jayasri 1 , B. Sampath Kumar 1* , G.V. Narasa Reddy 2 , V. Ravinder Reddy 3 1 Department of Veterinary Biochemistry, College of Veterinary Science, Korutla, Jagtiyal-505326., 2 Department of Veterinary Biochemistry, College of Veterinary Science, Rajendranagar, Hyderabad, 3 College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University. Rajendra nagar, Hyderabad, Telangana * Corresponding author Email: samkum85@gmail.com. Ph: +919989673085 Journal of Livestock Science (ISSN online 2277-6214) 8: 172-175 Received on 11/5/2017; Accepted on 16/7/2017 Abstract The present study was undertaken to study the combined effect of AF (Aflatoxin) and OA (Ochratoxin A) on kidney function parameters in broilers and their amelioration using adsorbents. AF and OA were mixed with the broiler diet to attain a concentration 1 ppm and 2 ppm respectively. Activated charcoal @0.4% level and lyophilized yeast culture@0.2 % level were selected for testing their efficacy in ameliorating the combined toxicity in broiler chicks. Total of 128 no., one day old broiler chicks were divided into 4 groups with 4 replicates of 8 birds each in a completely randomized design. Each group was fed with 4 different diets for 6 weeks to study the effect on kidneys function parameters. Group 1 is toxin free basal diet (Control). Group 2 is a mixture of basal diet, AF and OA. Group 3 is a mixture of basal diet, AF, OA and activated charcoal. Group 4 is a mixture of basal diet, AF, OA, activated charcoal and lyophilized yeast culture. The birds in group 2 recorded significantly higher serum uric acid and lower BUN level compared to control group. The birds in group 3 recorded significant improvement in kidney function parameters as compared to group 2 but still significantly varying from groups 4 and 1, indicating that activated charcoal @ 0.4.% level has only partial ameliorating effect on combined toxicity. Birds in group 4 have shown significant improvement in the kidney function parameters as compared to group 2 and 3 but there is still a significant difference from the control group. From the results it is observed that the addition of activated charcoal @0.4% has only partial ameliorating effect on combined toxicity where as a combination of activated charcoal @0.4% and yeast culture @ 0.2 % has complimentary effect in ameliorating the combined toxicity in broilers. However, combination of activated charcoal and yeast culture also could not completely ameliorate the combined toxicity in broilers. Key words: Aflatoxin; Ochratoxin A; Activated charcoal; Yeast culture; Kidney function parameters; Broilers