International Journal of Sciences: Basic and Applied Research (IJSBAR) ISSN 2307-4531 (Print & Online) http://gssrr.org/index.php?journal=JournalOfBasicAndApplied --------------------------------------------------------------------------------------------------------------------------- 154 Preventive and Curative Effects of Phyllanthus niruri Allium sativum Combination on Tiger Grouper Epinephelus fuscoguttatus Infected by Vibrio alginolyticus Dinamella Wahjuningrum a* , Shavika Miranti b , Irzal Effendi c a,c Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Jalan Agatis, Dramaga Campus, Bogor 16680, Indonesia b Study Program of Aquaculture, Graduate School, Dramaga Campus, Bogor 16680, Indonesia a Email: dinamellawa@ipb.ac.id b Email: shavika.miranti@gmail.com c Email: irzalef@gmail.com Abstract The aim of this study was to evaluate the preventive and curative effects of supplementation of the combination of stone-breaker (Phyllanthus niruri) and garlic (Allium sativum) through the feed to control bacterial diseases on tiger grouper juvenile (Epinephelus fuscoguttatus) caused by Vibrio alginolyticus. The extract was prepared in five combination doses with two replications: 15+20, 15+25, 20+20, 20+25 and 25+20 g L -1 . These combination doses were tested at in vitro test to observe the antibacterial activity of medicinal plants against V. alginolyticus (10 4 CFU mL -1 , 0.1 mL). The in vivo test was conducted using CRD (completely randomized design) consisting of four treatments and three replications; negative control, positive control, preventive, and curative treatment. Tiger grouper juveniles (length 7.74±0.27 cm; weight 12.50±0.394 g) were reared in aquariums (60x30x30 cm 3 ) with a stocking density of 6 fish per aquarium. The fish were fed with the experimental feed using at satiation method three times a day. ------------------------------------------------------------------------ * Corresponding author. brought to you by CORE View metadata, citation and similar papers at core.ac.uk provided by GSSRR.ORG: International Journals: Publishing Research Papers in all Fields