Conclusion: Our results suggest that both desaturation and dura- tion of apnea influence the amplitude of diastolic blood pressure and heart rate, without any impact on systolic blood pressure. http://dx.doi.org/10.1016/j.sleep.2013.11.651 Sleep disorders diagnosis by genetic assessment A. Martins-Da-Silva 1 , J. Ramalheira 2 , L. Silva 3 , D. Cunha 4 , S. Brás 5 , C. Carvalho 5 1 Serviço Neurofisiologia and UMIB/ICBAS, Hospital Santo António/CH Porto and ICBAS/University of POrto, Portugal 2 Serviço Neurofisiologia, Hospital Santo António/CH POrto, Portugal 3 Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, CESPU, Portugal 4 Laboratorio Imunogenética, ICBAS-Universidade do Porto, Portugal 5 Laboratório de Imunogenética, UMIB/ICBAS-Universidade do Porto, Portugal Introduction: Diagnosis of Sleep Disorders (SD) is frequently a dif- ficult task. Environmental and genetic factors influence the clinical SD phenotype masking its correct identification. The definition of such factors will help the physicians to easily classify SD. Accord- ingly a better treatment for such disorders should be obtained. The objective of the present work is to identify, on a cohort of SD patients, the possible genetic factors involved. Materials and methods: Four hundred and twenty-one SD patients were assessed at the Out Patient Sleep Clinic of Hospital Santo Antó- nio/CH Porto – by means of clinical history, physical and neurological evaluation, night sleep polygraphic EEG-Video recording (EEG, EOG, EMG – chin and L Limbs; Respiratory effort; O 2 saturation; EKG; snor- ing). For Narcolepsy without (N) or with Cataplexy (NC) and for Hyper- somnia (H) qualification/quantification a MSLT was performed on the day after night registration. Blood venous sample was taken after informed consent. Ethical approval was obtained for research studies. For the purpose of distinction between diverse Sleep Disorders HLA– DRB1 / was genotyped using a PCR-Sequence Specific Primer (SSP) methodology. A control population (223 individuals) was matched to age, gender, ethnicity and geographical origin. Statistical analysis was performed and the phenotypic frequencies were evaluated by chi- square of 2 Â 2 contingency tables. Odds Ratios and their respec- tive 95% confidence interval were calculated. Values of p < 0.05 were considered as statistically significant. Results: From the 421 SD patients studied 302 were classified as obstructive sleep apnea syndrome (OSAS); 64 as H; 37 NC and 16 N. Significant values (p < 0.05) of HLA–DRB1 / alelles were found to OSAS: HLA-DRB1 / 09 (1% OSAS vs. 6% PC); NC: HLA-DRB1 / 13 (14% NC vs. 30% PC) and 15 (76% NC vs. 21% PC); N: HLA-DRB1 / 15 (44% N vs. 21% PC); H: HLA–DRB1 / 03 (33% H vs. 15% PC). Conclusion: Our studies emphasize that the HLA–DRB1 / genetic characterization of SD patients may identify different profiles. The results point out to the identification of susceptibility alleles, HLA– DRB1 / 15 for N and NC and HLA–DRB1 / 03 for Hypersomnia. The fre- quencies of HLA–DRB1 / 09 in OSAS and of HLA–DRB1 / 13 in NC indi- cate that these alleles may act as protective factors. These findings point to the value of immunogenetic approach to a better under- standing of the pathophysiology of SD and to a better classification of patients with sleep disorders. Acknowledgement: / Studies on SAOS were granted by a CESPU Project. http://dx.doi.org/10.1016/j.sleep.2013.11.652 Incidence and remission of sleep related symptoms in children and associations with health-related quality of life; A 7-year follow-up of the TuCASA cohort G. Silva 1 , J. Goodwin 1 , K. Archibald 1 , M. Vasquez 1 , S. Quan 2 1 University of Arizona, United States 2 Harvard Medical School, United States Introduction: Sleep disturbances are common in adolescents; some studies have shown that this may adversely affect their quality of life. However, there is little evidence on incidence and remission of sleep problems from childhood to adolescence and the association with health-related quality of life (QoL). Materials and methods: Sleep Screening questionnaires were col- lected on Hispanic and Caucasian children 6–12 years of age at baseline, and again 7 years after during follow-up, when partici- pants were 12–20 years. Excessive daytime sleepiness (EDS) and difficulty initiating and maintaining sleep (DIMS) were present if they occurred frequently or more. Health-related QoL was assessed at follow-up using the Pediatric Quality of Life Inventory and three summary scores, ranging from 1 to 100, were evaluated: psychosocial health (PS), physical function (PF), and total scale scores (TS). Prevalence, incidence, and remission of sleep symptoms were computed and analyzed for association with health-related QoL. Results: The mean ages at baseline and follow-up were 8.8 and 15.4 years. There were 50.2% males and 30.6% Hispanics. Incidence rates of EDS and DIMS were 39.1% and 58.9%, and remission rates were 53.1% and 36.2%, respectively. Separate linear regressions models predicting health-related QoL showed that participants with persistent EDS had lower mean values for TS (coeff. = À9.2, p = .006), PS (coeff. = À8.8, p = .025), and PF (coeff. = À10, p = .002) as compared with participants who never had EDS or those with remittent EDS combined. Participants with persistent DIMS had lower mean values for TS (coeff. = À6.8, p = .001) and PS (coeff. = À8.4, p = .001) as compared with participants who never had DIMS or those with remittent DIMS combined. When partici- pants with persistent DIMS were compared with those with remittent DIMS only, the associations between DIMS and health-related QoL, became non-significant. However, the associ- ation between EDS and health-related QoL remained (coeff. = À13.3, p = .02). Conclusion: Substantial variability of self-reports of sleep prob- lems exists as children age from childhood to adolescence. Neverthe- less, there are a small number of young children who persistently have sleep problems over this age span and these problems are asso- ciated with lower health-related quality of life. These associations appear to be driven primarily by EDS. Acknowledgements: The TuCASA study was supported byNHLBI grant HL 62373. Silva, G.E. was supported by NHLBI grant HL 62373-05A2S1. http://dx.doi.org/10.1016/j.sleep.2013.11.653 Predictive power of HLA-DQB1 / to identify narcoleptic patients M. Silva 1 , J. Lopes 2 , C. Carvalho 1 , D. Cunha 1 , P. Pinho Costa 3 , A. Martins-Da-Silva 4 1 Laboratório Imunogenética, UMIB/ICBAS, Portugal 2 Serviço Neurofisiologia, Hospital Santo António/CH Porto, Portugal 3 4Instituto Nacional Saúde Ricardo Jorge/Porto and UMIB/ICBAS, 4Instituto Nacional Saúde Ricardo Jorge/Porto, Portugal 4 Serviço Neurofisiologia and UMIB/ICBAS, Hospital Santo António/CH Porto and UMIB/ICBAS-University of Porto, Portugal e268 Abstracts / Sleep Medicine 14S (2013) e239–e317