Published by Advance Educational Institute and Research Centre International Journal of Endorsing HEALTH SCIENCE RESEARCH Study Protocol Association of genetic variant of OXTR (rs53576) and MTNR-1B (rs10830963 and rs1387153) with symptoms of stress in Pakistani women with Gestational Diabetes. Fatima Abid 1 , Sadaf Ahmed 2 , Syeda Sadia Fatima 3 & Shamoon Noushad 4 1 Department of Physiology, Jinnah Sindh Medical University, Karachi-Pakistan. 2 Department of Physiology, University of Karachi, Karachi-Pakistan. 3 Department of Biological and Biomedical Sciences, Aga Khan University, Karachi-Pakistan. 4 Department of Health Physical Education & Sports Sciences, University of Karachi, Karachi-Pakistan. Abstract Background: Pregnancy continues to be an event full of risks, complications and adverse outcomes for women despite advanced clinical and diagnostic methods and treatment options available in medicine. Diabetes, as a multifactorial and complex disease, is a possible outcome of a blend of numerous genetic variants of differing scarcities. The exact etiology of gestational diabetes mellitus (GDM) is unclear; however, the interaction of genetic and environmental factors, especially the genetic variants identified as the genetic loci for type 2 diabetes as candidate genes for GDM. Methodology: It will be a case-control study with a genetic analysis of single nucleotide polymorphisms (SNPs) of candidate genes expressed using real-time PCR with sequencing. The study will also evaluate the primer sequence of SNPs. Discussion: We will be able to share the findings after the completion of our research. The results of this study will able helpful in addressing the psycho-physiological needs of women with GDM, which may also be used to devise strategies to benefit the sustainable long-term behavioural change following the affected pregnancy. Keywords Gestational Diabetes Mellitus, Stress, OXTR, MNTR1B, Single Nucleotide Polymorphism. Doi: 10.29052/IJEHSR.v9.i3.2021.420-425 Corresponding Author Email: fatima.abid@jsmu.edu.pk Received 02/06/2021 Accepted 05/08/2021 First Published 01/09/2021 © The Author(s). 2021 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)