βeta-glucan synthase gene expression in Pleurotus sp. Azhar Mohamad 1 , Abdul Rahman Zulkiflli 2 , Zaiton Abdul Kadir 2 , Wan Safina Wan Abdul Razak 2 , Fauzi Daud 2 , Mohd Shazrul Fazry Sa’ariwijaya 2 , Hing Jan Nie 1 , Ibrahim Mahmood 2 , Hisham Hamza Hussain 2 Bioprocess Group, Agrotechnology and Biosciences Division, Malaysian Nuclear Agency, Bangi 43000, Selangor, Malaysia. School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600 Selangor, Malaysia. Abstract Pleurotus sp. is a popular edible mushroom, containing various functional component, in particular, βeta-glucan. βeta-glucans is a part of glucan family of polysaccharides and supposedly contribute to medicinal and nutritional value of Pleurotus.sp. In order to understand the distribution of βeta-glucan in Pleurotus.sp, the βeta-glucan synthase gene expression was determined and compared in different part of Pleurotus, namely mycelium, stipe and cap. The Pleurotus.sp RNA was extracted using commercial kit, employing Tissuelyser ll (Qiagen, USA) to disrupt the cell walls. Then the RNA was quantified by Nanodrop (ThermoFisher, USA) and visualized using denaturating agarose gel. RNA with good OD 260⁄280 reading (≈2.0) was chosen and converted to cDNA. Using Laccase synthase gene as homekeeping gene, βeta-glucan synthase gene expression was quantified using CFX 96 Real Time PCR detection system (Biorad, USA). Preliminary result shows that βeta-glucan synthase was relatively expressed the most in stipe, followed by mycelium and barely in cap. Katakunci/keyword : gene expression, Beta Glucan, cloning