A.C. Estalote et al. 166 Genetics and Molecular Research 4 (2): 166-173 (2005) www.funpecrp.com.br The mutation G298A→Ala100Thr on the coding sequence of the Duffy antigen/ chemokine receptor gene in non-caucasian Brazilians Angela Cristina Estalote 1 , Rodrigo Proto-Siqueira 2,4 , Wilson Araújo Da Silva Jr. 3,4 , Marco Antonio Zago 2,4 and Marcos Palatnik 1 1 Departamento de Clínica Médica, Hospital Universitário Clementino Fraga Filho, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil 2 Departamento de Clínica Médica, 3 Departamento de Genética, Faculdade de Medicina, Universidade de São Paulo, Ribeirão Preto, SP, Brasil 4 Centro de Terapia Celular, Ribeirão Preto, SP, Brasil Corresponding author: M. Palatnik E-mail: palatos@infolink.com.br Genet. Mol. Res. 4 (2): 166-173 (2005) Received August 10, 2004 Accepted February 14, 2005 Published April 29, 2005 ABSTRACT. Ala100Thr has been suggested to be a Caucasian ge- netic marker on the FY*B allele. As the Brazilian population has arisen from miscegenation among Portuguese, Africans, and Indians, this mu- tation could possibly be found in Euro- and Afro-Brazilians, or in Brazil- ian Indians. Fifty-three related individuals and a random sample of 100 subjects from the Brazilian population were investigated using the poly- merase chain reaction and four restriction fragment length polymorphisms. Confirming the working hypothesis, among the related individuals three Afro-Brazilians (two of them a mother and daughter) and a woman of Amerindian descent had the Ala100Thr mutation on the FY*B allele. Five non-related Euro-Brazilians also carried the mutation. All nine indi- Genetics and Molecular Research 4 (2): 166-173 (2005) FUNPEC-RP www.funpecrp.com.br