s1 1 SUPPORTING INFORMATION 2 Flow Water Proton NMR: In-Line Process Analytical Technology For Continuous 3 Biomanufacturing 4 Marc B. Taraban, Katharine T. Briggs, Peter Merkel, Y. Bruce Yu* 5 1 Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, 6 Maryland 21201 7 8 TABLE OF CONTENTS 9 Table S1. Aggregate content in stressed protein solutions ..............................................................s2 10 Spin residence time in the magnet ......................................................................................................s3 11 Figure S1. Cylindrical PTFE cells for high and low flow rates ......................................................s3 12 Spin magnetization in the prepolarization zone ................................................................................s4 13 Figure S2. Dependences of R 2 ( 1 H 2 O) on pH and conductivity under flow conditions ..................s5 14 Figure S3. Dependences of R 2 ( 1 H 2 O) on protein concentration under flow conditions ................s6 15 Table S2. Slopes of linear dependences of R 2 ( 1 H 2 O) vs. flow rates ................................................s7 16 Table S3. Slopes of linear dependences of R 2 ( 1 H 2 O) vs. protein concentrations ...........................s7 17 Figure S4. 3D combined effects of concentration and flow rate on R 2 ( 1 H 2 O) ...............................s8 18 Figure S5. Dependences of R 2 ( 1 H 2 O) on aggregate content under flow conditions .......................s9 19 Figure S6. 3D combined effects of aggregate content and flow rate on R 2 ( 1 H 2 O) .........................s10 20 21