References [1] E. Martines, M. Zuin, R. Cavazzana, et al., New J. Phys, 11, 115014 (2009). [2] Paola Brun, Surajit Pathak, Ignazio Castagliuolo, Giorgio Palù, Paola Brun, Matteo Zuin, Roberto Cavazzana, Emilio Martines, PLOS ONE 9, e104397 (2014). [3] W. Y. Hung, K. H. Huang, C. W. Wu, C. W. Chi, H. L. Kao, A. F. Li, P. H. Yin, H. C. Lee, Biochim. Biophys. Acta 1820, 1102 (2012). E-mail address: emilio.martines@igi.cnr.it (E. Martines). http://dx.doi.org/10.1016/j.cpme.2017.12.045 Where Do Ros Go? Oxidation Cascades In Melanoma Exposed To Cold Physical Plasma Katrin Rödder, Rajesh Gandhirajan, Thomas von Woedtke, Sander Bekeschus Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK plasmatis, Felix-Hausdorff- Str. 2, 17489 Greifswald, Germany In plasma medicine, physical atmospheric cold plasma is used for application in therapeutic appliances such as wound healing or decontamination of infected skin.[1] Over the last decade, plasmas became a valuable research tool for cancer therapy.[2] Many groups have not only reported an efficient killing of cancer cells with plasma but also a selectivity of the killing of malignant over non-malignant cells.[3] Yet, following the trajectories of plasma- derived reactive oxygen and nitrogen species has only begun to be explored. First modeling studies suggest an important role of lipid peroxidation and phosphatidylserine content in the cell membrane.[4] Using fluorescent reporter dyes, many groups have reported cytosolic oxidation following plasma treatment. Some groups claim that DNA double-stranded breaks are a direct product of reactive species deposition by plasmas.[5] Using human mela- noma cells, the purpose of this work was to study in detail how different cell compartments are affected reactive species derived the atmospheric pressure argon plasma jet kINPen. Using confocal laser scanning microscopy, several versions of fluorescent reporter dyes were used to assess oxidation qualitatively and quantitatively. We hope our study to contribute to the understanding how plasma-generated reactive species penetrate cells and affect their behavior and viability, especially for future plasma cancer treatment. This work was supported by the German Federal Ministry of Education and Research, grant number 03Z22DN11. References [1] S. Bekeschus, A. Schmidt, KD Weltmann, T. von Woedtke, Clinical Plasma Medicine, 4, 19-28 (2016). [2] D. Yan, JH Sherman, M. Keidar, Oncotarget, 8(9), 15977-15995 (2017). [3] K. Panngom, KY Baik, MK Nam, JH Han, H Rhim, EH Choi, Cell Death and Disease, 4, e643 (2013). [4] J. Van der Paal, EC Neyts, CCW Verlackt, A. Bogaerts, Chemical Science, 7, 489-498 (2016). [5] Y. Lee, K. Kim, K. Kang, J. Lee, S. Yang, W. Chung, Archives of Biochemistry and Biophysics, 560, 1-9 (2014). E-mail address: sander.bekeschus@inp-greifswald.de (S. Bekeschus). http://dx.doi.org/10.1016/j.cpme.2017.12.046 Cold Atmospheric Plasma Selectively Induces Apoptosis In Renal Adenocarcinoma Warren J Rowe III 1,5 , Xiaoqian Cheng 1,5 , Lawan Ly 1 , Giacomo Basadonna 1,2 , Barry Trink 1,3 , Michael Keidar 1,4 , Jerome Canady 1 1 Jerome Canady Research Institute for Advanced Biological and Technological Sciences, Takoma Park, MD, USA 2 University of Massachusetts School of Medicine, Worcester, MA, USA 3 Department of Otolaryngology, Rambam Medical Center, Haifa, Israel 4 The George Washington University, Washington DC, USA The application of cold atmospheric plasma (CAP) in the selective treatment of cancer has drawn increasing attention. However, very few studies have focused on the selective effect of CAP towards cancer compared to the corresponding normal cells, among which the results have been mixed. Guerrero- Preston has shown that CAP selectively kills head and neck squamous cell carcinoma. While the normal oral cavity epithelial cells were killed to some degree, the mechanism of selectivity was not reported [1]. Breast cancer has been shown to be selectively killed relative to mesenchymal stem cells (MSCs) [2], however, no selectivity was found when compared to normal breast epithelial cells [3]. CAP induces apoptosis of lung cancer without affecting bronchial epithelial cells, but no apoptotic pathways were identified or proposed [4]. Ishaq et al. proposed NADPH oxidase (Nox2) as the endogenous ROS 1 The authors have contributed equally. Clinical Plasma Medicine 9 (2018) 2–48 29