Journal of Chromatography B, 815 (2005) 147–155 Review Proteomic analysis of Korean ginseng (Panaxginseng C.A. Meyer) Myung Hee Nam a , Seung Il Kim b , Jang Ryol Liu c , Deok Chun Yang d , Yong Pyo Lim e , Kyung-Hoon Kwon b , Jong Shin Yoo b , Young Mok Park b,∗ a MetabolomeAnalysisTeam,KoreaBasicScienceInstitute,Seoul136-701,Korea b ProteomeAnalysisTeam,KoreaBasicScienceInstitute,Daejeon305-333,Korea c LaboratoryofPlantCellBiotechnology,KoreaResearchInstituteofBioscienceandBiotechnology(KRIBB), Daejeon305-333,Korea d CollegeofLifeScience,Kyung-HeeUniversity,Suwon449-701,Korea e DepartmentofHorticulture,ChungnamNationalUniversity,Daejeon305-764,Korea Received 10 October 2004; accepted 20 October 2004 Available online 8 December 2004 Abstract Although many reports have been published regarding the pharmacological effects of ginseng, little is known about the biochemical pathways operant in ginsenoside biosynthesis, or the genes involved therein. Proteomics analysis is an approach to elucidate the physiological characteristics and biosynthetic pathways of ginsenosides, main components of ginseng. In this review, we introduced the recent progress in proteomics studies of ginseng (PanaxginsengC.A.Meyer). We briefly reference the genomic analyses of P.ginseng, without which proteomics approaches would have been impossible. Functional genomics studies regarding secondary metabolism in P.ginseng are also introduced here, in order to introduce possible future prospects for further study. © 2004 Elsevier B.V. All rights reserved. Keywords: Panaxginseng; Proteomics; Araliaceae Contents 1. Introduction ..................................................................................................... 148 2. Genomics of P.ginseng ........................................................................................... 148 2.1. BAC-end sequencing project ................................................................................ 148 2.2. EST sequencing project ..................................................................................... 149 3. Proteomics of P. ginseng .......................................................................................... 149 3.1. Proteomic challenges of P. ginseng ........................................................................... 149 3.1.1 Sample preparation for 2DE ......................................................................... 149 3.1.2 Databases ......................................................................................... 149 3.1.3 Selection of appropriate tissue ....................................................................... 150 3.2. Proteomics studies in P.ginseng ............................................................................. 150 3.2.1 Proteome analysis of wild root and cultured hairy root ................................................. 150 3.2.2 High light-induced proteome in leaves ................................................................ 151 3.2.3 Applications of proteome analysis ................................................................... 152 ∗ Corresponding author. Tel.: +82 42 865 3420; fax: +82 42 865 3419. E-mailaddress: ympark@kbsi.re.kr (Y.M. Park). 1570-0232/$ – see front matter © 2004 Elsevier B.V. All rights reserved. doi:10.1016/j.jchromb.2004.10.063