animals
Article
Evaluation of the Safety and Feasibility of Apheresis in Dogs:
For Application in Metastatic Cancer Research
Haru Yamamoto
1,†
, Mohamed Elbadawy
1,2,†
, Koudai Fujisaka
1
, Yomogi Sato
1
, Takahiro Ohmori
3
,
Yuta Shinohara
1,4
, Yui Hatano
5
, Daichi Kobayashi
5
, Ayana Gomyo
5
, Yuji Sudo
5
, Daigo Azakami
5,
* ,
Tsuyoshi Uchide
6
, Ryuji Fukushima
3
, Shohei Morita
3
, Amira Abugomaa
1,7
, Hideyuki Yamawaki
8
,
Masahiro Kaneda
9
, Tatsuya Usui
1,
* and Kazuaki Sasaki
1
Citation: Yamamoto, H.; Elbadawy,
M.; Fujisaka, K.; Sato, Y.; Ohmori, T.;
Shinohara, Y.; Hatano, Y.; Kobayashi,
D.; Gomyo, A.; Sudo, Y.; et al.
Evaluation of the Safety and
Feasibility of Apheresis in Dogs: For
Application in Metastatic Cancer
Research. Animals 2021, 11, 2770.
https://doi.org/10.3390/ani11102770
Academic Editors: Irina Amorim and
Marian Taulescu
Received: 19 July 2021
Accepted: 18 September 2021
Published: 23 September 2021
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4.0/).
1
Laboratory of Veterinary Pharmacology, Cooperative Department of Veterinary Medicine,
Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu,
Tokyo 183-8509, Japan; s206628w@st.go.tuat.ac.jp (H.Y.); mohamed.elbadawy@fvtm.bu.edu.eg (M.E.);
s162840t@st.go.tuat.ac.jp (K.F.); s202090x@st.go.tuat.ac.jp(Y.S.); y.shinohara@iskra.co.jp (Y.S.);
s193249s@st.go.tuat.ac.jp (A.A.); skazuaki@cc.tuat.ac.jp (K.S.)
2
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor,
Toukh 13736, Elqaliobiya, Egypt
3
Animal Medical Center, Faculty of Agriculture, Tokyo University of Agriculture and Technology,
3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan; tkhromori5@gmail.com (T.O.); ryu-ji@cc.tuat.ac.jp (R.F.);
fq7087@go.tuat.ac.jp (S.M.)
4
Pet Health & Food Division, Iskara Industry Co., Ltd., 1-14-2, Nihonbashi, Chuo-ku, Tokyo 103-0027, Japan
5
Laboratory of Clinical Oncology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture,
Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan;
s160384s@st.go.tuat.ac.jp (Y.H.); s167504x@st.go.tuat.ac.jp (D.K.); s179850u@st.go.tuat.ac.jp (A.G.);
s170620w@st.go.tuat.ac.jp (Y.S.)
6
Laboratory of Veterinary Molecular Pathology and Therapeutics, Cooperative Department of Veterinary
Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu,
Tokyo 183-8538, Japan; uchide@cc.tuat.ac.jp
7
Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Dakahliya, Egypt
8
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, 35-1,
Higashi 23 Ban-cho, Towada, Aomori 034-8628, Japan; yamawaki@vmas.kitasato-u.ac.jp
9
Laboratory of Veterinary Anatomy, Cooperative Department of Veterinary Medicine, Faculty of Agriculture,
Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan;
kanedam@cc.tuat.ac.jp
* Correspondence: azakami@go.tuat.ac.jp (D.A.); fu7085@go.tuat.ac.jp (T.U.); Tel.: +81-42-367-5739 (D.A.);
+81-42-367-5770 (T.U.); Fax: +81-42-367-5739 (D.A.); +81-42-367-5770 (T.U.)
† These authors contributed equally to this work.
Simple Summary: In cases of cancer metastasis, some tumor cells circulate in blood (CTCs) and
function as a seed for metastasis. These cells are important for detailed study of the molecular basis
of metastasis. However, their few numbers and difficult isolation hinder its analysis. Apheresis,
a process used to isolate a specific component of blood, can efficiently separate peripheral blood
monocyte (PBMC), whose density is closely like that of CTCs. In the present study we used the dogs
as a precious model of human’s cancers to check the safety and feasibility of apheresis and to separate
PBMC and infused MCF7 cells using Spectra Optia apheresis machine (Terumo). The process was
performed safely to capture PBMC and MCF7. The captured MCF7 cells were regrown up in vitro
and characterized. In conclusion, Spectra Optia apheresis machine (Terumo) can be used safely to
isolate CTCs from dogs blood with precautions to keep hemodynamic stability.
Abstract: In patients with solid tumors, circulating tumor cells (CTCs) spread in their blood and
function as a seed for metastases. However, the study of CTCs has been limited by their rarity, low
frequency, and heterogeneity. The efficient collection of CTCs will contribute to further research of
metastatic cancers. Apheresis is a process in which the whole blood of an individual is passed through
a machine that isolates a particular constituent and returns the remainder to the circulation. In the
present study, we investigated the safety and feasibility of apheresis to separate peripheral blood
monocytes (PBMCs), whose density is closely similar to that of CTCs, and to capture intravenously
Animals 2021, 11, 2770. https://doi.org/10.3390/ani11102770 https://www.mdpi.com/journal/animals