Vol.:(0123456789) 1 3
Journal of Inorganic and Organometallic Polymers and Materials
https://doi.org/10.1007/s10904-020-01549-y
COMMUNICATION
Rapid Green Synthesis of Silver Nanoparticles Using Ethiopian
Cactus Pear Fruit Peel Infusions and Evaluation of Its In Vitro Clinical
Potentials
M. Kamaraj
1,2
· T. G. Nithya
3
· P. Santhosh
3
· Kidist Mulugeta
1
Received: 8 March 2020 / Accepted: 16 April 2020
© Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
In the current study, the Ethiopian cactus pear fruit peel infusions were used as a phytoreducing agent to fabricate silver nano-
particles (CPFP-AgNPS). The formation of AgNPS in 7 min was confrmed by Ultra UV–Vis spectral portrait. The average
size range (20–60 nm), the composition of silver element and crystal structure of the CPFP-AgNPS was established by trans-
mission electron microscopy, energy dispersive X-ray spectra and X-ray difraction analysis respectively. The CPFP-AgNPS
exhibited greater inhibition (81.92%) on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay. The antibacterial activity of
CPFP-AgNPS was high in the order against E.coli > Salmonella spp. > Bacillus spp. > Pseudomonas spp. > Staphylococcus
spp. respectively. The CPFP-AgNPS also showed efective anticancer activity against human cervix epithelioid carcinoma cell
lines with an IC
50
value of 74.26 ± 0.25 µg mL
−1
/24 h. In short, the fndings highlighted the efectiveness of CPFP-AgNPS
on potential therapeutic applications.
Keywords Green chemistry · Functional material · Nanoparticles · Pharmacology
1 Introduction
Silver nanoparticles (AgNPs) are receiving a unique focus
on nanotechnology research due to their wide range of appli-
cations in medicine, catalysis, optics. Phytochemical medi-
ated synthesis of AgNPS is a green approach (safe and eco-
friendly) to replace the chemical reductants, which are used
in the traditional chemical reduction process and reduces
their hazards towards the environment. Several plants and
plants based materials are tested as a substrate for the syn-
thesis of Ag-NPs due to their reduction potential and simple
scale-up process [1]. Cactus pear (Opuntia ficus-indica) are
grouped under the Cactaceae family, which is locally known
by the vernacular name of “Belles” in Ethiopia [2]. Cactus
pear wastages can be very well harnessed and it is one of
the most important productive plants per unit of a cultivated
feld. The exploitation of the non-edible part of this fruit as a
cheap source of substrate for AgNPs synthesis applications
is an evolving movement that entails an imperative reduction
in the generation of waste. Furthermore, it reconnoiters the
efciency of cactus pear fruit peel (CPFP) on useful avenues.
Compared with other methods, plant infusion mediated
synthesis of nanoparticles is promising in favor of eco-
friendly, immediacy, and high efciency [3]. So, the fnd-
ing of new plant sources for nanoparticle synthesis is still
in search. Hence, the current study aimed (i) to synthesize
AgNPs using Ethiopian CPFP as a reducing agent, (ii) to
characterize the phytosynthesized AgNPs, (iii) to evaluate
their therapeutic property (antibacterial, antioxidant and
anticancer) under in vitro conditions.
* M. Kamaraj
drkamarajm@gmail.com
1
Department of Biotechnology, College of Biological
and Chemical Engineering, Addis Ababa Science
and Technology University, 16417 Addis Ababa, Ethiopia
2
Nanotechnology Center of Excellence, Addis Ababa Science
and Technology University, 16417 Addis Ababa, Ethiopia
3
Department of Biotechnology, FSH, SRM Institute
of Science and Technology, Kattankulathur,
Tamil Nadu 603203, India