CHEMIJA. 2009. Vol. 20. No. 3. P. 154–161
© Lietuvos mokslų akademija, 2009
© Lietuvos mokslų akademijos leidykla, 2009
Investigation of electrode kinetics and thermodynamics
of [Zn–L-amino acidate–vitamin B
7
] complexes by
voltammetric technique
Farid Khan*,
Afroza Khanam
Electrochemical Laboratory,
Department of Chemistry,
Dr. H. S. Gour University,
Sagar, M. P.,
India
* Corresponding author. E-mail: faridkhan58@yahoo.com;
farid.f@redifmail.com
Te voltammetric reduction of Zn
2+
using L-lysine, L-ornithine, L-threonine, L-serine, L-
phenylglycine, L-phenylalanine, L-glutamic acid, L-aspartic acid and vitamin B
7
(biotin) at
pH 7.30 ± 0.01 and I = 1.0 M NaClO
4
was reported at 25 and 35 °C. Te nature of the current
voltage curves was quasireversible and difusion-controlled. Zn
2+
formed 1 : 1 : 1, 1 : 1 : 2
and 1 : 2 : 1 complexes with these drugs as confrmed by the Schaap and McMaster method.
Te sequence of the stability constant of L-lysine < L-ornithine < L-threonine < L-serine
< L-phenylglycine < L-phenylalanine < L-glutamic acid < L-aspartic acid complexes can
be explained on the basis of the size, basicity and steric hindrance of ligands. Te values of
the stability constant (log β) varied from 2.13 to 11.37 and confrm that these drugs, i. e.
L-amino acids or in combination with vitamin B
7
or their complexes, could be used against
Zn
2+
toxicity. Te thermodynamic parameters such as enthalpy (∆H), free energy (∆G) and
entropy change (∆S), are also reported. Te kinetic parameters viz. the transfer coefcient
(α), degree of irreversibility (λ), difusion coefcient (D) and standard rate constant (k)
were calculated. Te values of α confrmed the symmetric nature of the ‘activated complex’
between oxidants and reductants in response to the applied potential between the drop-
ping mercury electrode and solution interface.
Key words: voltammetry, thermodynamic parameters, electrode kinetics, [Zn–L-amino
acidate–vitamin B
7
] complexes
INTRODUCTION
Most of L-amino acids are blood plasma ligands that form
stable complexes with various essential metals in vivo [1] and
play an important role in biology, pharmacy and industry
[2–4]. Complexes of some metal ions with amino acids can
be used as models to study the pharmaco-dynamic efects of
drugs or for increasing the biocompatibility and minimizing
the toxic efects of some metal ions [5]. On the other hand, L-
amino acids are also involved in intracellular metabolism and
operate specifc transport systems of the plasma membrane;
they do not afect cardiac function under normal conditions
[6, 7]. Te invention provides the use of zinc complexes of
selected amino acids and other pharmacologically acceptable
salts of zinc. Te use of the compound comprises adminis-
tering an efective amount of said compounds for inhibiting
the growth of the malarial parasite, plasmodium falciparum
[8]. Vitamin B
7
(biotin or vitamin H) [9] is a member of the
vitamin B complex and water-soluble. Biotin is involved in
energy metabolism and plays a role in enabling the body to
use glucose [10]. Biotin is sometimes chemically linked to a
molecule or protein for biochemical assays [11] and earned a
keen attention towards the studies of their metal complexes.
Terefore, Zn complexes of these drugs are of great impor-
tance. Te concentrations of zinc in vivo can be reduced by
drug therapy, but the specifcity of the drug and its amount
are stability-constant-dependent [12]. Terefore, the authors
have undertaken the present study to determine the stability
constants, thermodynamic and kinetic parameters of ternary
complexes with these selected drugs for which no reference
has been traced out so far in the literature.
EXPERIMENTAL
Instrumentation
Te electrochemical experiment, i. e. a simple DC polaro-
graphy, was carried out using a manual polarograph with