Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Fri, 07 Dec 2018 07:32:06 Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave Judit Makk, 1 Erika M. Tóth, 1 Dóra Anda, 1 S ara P al, 1 Peter Schumann, 2 Attila L. Kov acs, 3 Judit M adl-Szo }nyi, 4 K aroly M arialigeti 1 and Andrea K. Borsodi 1 Correspondence Judit Makk makkjudit@gmail.com 1 Department of Microbiology, Eötvös Lor and University, P azm any P. s et any 1/C, H-1117 Budapest, Hungary 2 DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany 3 Department of Anatomy-, Cell- and Developmental Biology, Eötvös Lor and University, P azm any P. s et any 1/C, 1117 Budapest, Hungary 4 Department of Physical and Applied Geology, Eötvös Lor and University, P azm any P. s et any 1/C, 1117 Budapest, Hungary Following the exposure of a biofilm sample from a hydrothermal spring cave (Gell ert Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38 T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0–9.0, with an optimum at pH 7.0. The temperature range for growth was 20–37  C, with an optimum at 28  C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140 T (94.1 %), Deinococcus aquaticus PB314 T (93.3 %) and Deinococcus aerophilus 5516T-11 T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C 16 : 0 and C 16 : 1 !7c, and the cell- wall peptidoglycan type was A3b L-Orn–Gly 2-3 , corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38 T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D 10 ) of strain FeSTC15-38 T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38 T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38 T (=NCAIM B.02630 T =DSM 101791 T ). The genus Deinococcus was proposed by Brooks & Murray (1981) and, at the time of writing, comprised 59 species with validly published names (http://www.bacterio.net). Species of the genus Deinococcus are strictly aerobic, form coccoid or rod-shaped cells, colony colour ranges from yellow to pink, orange and red, and they are generally Gram-stain-positive (some strains are Gram-stain-negative) by a layered cell wall. They have L-ornithine in the cell-wall peptidoglycan and lack teichoic acids, and possess menaqui- none MK-8 as the major respiratory quinone. The majority of the members of the genus Deinococcus show high resis- tance to UV and gamma radiation and to desiccation (Rainey et al., 1997; Battista & Rainey, 2001). The Buda Thermal Karst System (BTK) is an active hypo- genic karst area where microbes participate in mineral pre- cipitation and in hydrothermal cave-forming processes as well (Borsodi et al., 2012). Microbial communities form, e.g. red iron-rich biofilm layers, and inhabit the partially water-filled caves at the discharge zone of the BTK. The red Abbreviation: D 10 , decimal reduction dose. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain FeSTC15-38 T is LN864925. Three supplementary figures and three supplementary tables are avail- able with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2016), 66, 5345–5351 DOI 10.1099/ijsem.0.001519 001519 ã 2016 IUMS Printed in Great Britain 5345