Int.J.Curr.Microbiol.App.Sci (2018) 7(2): 2210-2215 2210 Original Research Article https://doi.org/10.20546/ijcmas.2018.702.266 Comparative Study of the Nutritional Content of White Button Mushroom [Agaricus bisporus (Lange) Imbach] after Application of Pseudomonas putida Binit Kumar * , Chanchila Kumari and Manish Kumar Indian Council of Agricultural Research-National Rice Research Institute, Central Rainfed Upland Rice Research Station, Hazaribag, Krishi Vigyan Kendra, Koderma (Jharkhand), India *Corresponding author ABSTRACT Introduction Mushroom production is a growing business in India as the demand is increasing from last few years. It is a matter of practice and technical knowledge rather than labor intensive farming with a high value of return in short time. Mushroom production has some key raw materials to get good yields. Mushrooms and other fleshy fungi are the premier recyclers of nutrients on the planet (Stamets, 2000). Yet to date, the inherent biological power embodied within the mycelium network of mushrooms largely remains a vast, untapped resource. Water defines the biosphere, and low water availability in arid regions of the globe limits biodiversity as well as biomass (Austin et al., International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 02 (2018) Journal homepage: http://www.ijcmas.com The white button mushroom (Agaricus bisporus) is very popular throughout the world and is the most important mushroom of commercial significance in India. It can be successfully cultivated in places where the environmental conditions are favorable but it is cultivated in North India in winter seasons due to the favorable conditions. The optimum temperature for mycelial growth is 20°C -25°C and that for fruit body formation 15°C - 20°C and a high percentage of relative humidity (80-85%). The experiment was designed to evaluate the effect of bio-inoculants on the growth, yield and biochemical content of white button mushroom. Wheat straw supplemented with wheat bran was used for compost and application of Pseudomonas putida in casing layer was done by sprinkling of water. The casing combinations Garden Loam soil (GLS)+ Farm Yard Manure (FYM)+ Vermicompost (VC)+ Waste tea leaf + Pseudomonas putida (10 8 c.f.u./ml)was gave significant difference in all the parameters i.e. minimum days required for fruitification (10.3 days), number of fruiting bodies per bag (94.3), fresh weight (1.02kg), protein (0.34mg), carbohydrate (0.26mg), moisture content (89.5%) and biological efficiency (20.24%) in comparison to non-supplemented casing layer with same combination. This research was focused upon the evaluating the nutritional content of white button mushroom after addition of bio-inoculants as a bio-fertilizer in casing layer. Keywords Agaricus bisporus, Pseudomonas putida, Vermicompost and Waste tea leaf Accepted: 20 January 2018 Available Online: 10 February 2018 Article Info