Journal of Scientific & Industrial Research Vol. 76, March 2017, pp. 184-186 Biodiesel Waste Based New Generation Formulation of Permethrin for Cockroach Control M Pant 1 , N Kumar 1 , Ompal 1 , S Dubey 2 * and P K Patanjali 1 1 Institute of Pesticide Formulation Technology, Sector 20, Udyog Vihar, Gurgaon, India *2 Indian Institute of Technology, New Delhi, India Received 02 October 2015; revised 11 August 2016; accepted 21 November 2016 Permethrin Microemulsion, Nanoemulsion & Emulsifiable concentrate was formulated using biodiesel waste as an active dissolving solvent or as a carrier solvent. Process of stable emulsion development was optimized for parameters such as non-ionic surfactants concentration in micro and nanoemulsion and anionic surfactant concentration in emulsifiable concentration with high physical and chemical stability. Permethrin was analyzed by Gas chromatography using ECD and physical stability of emulsions was optimized in terms of shelf life of the product. The permethrin microemulsion (ME) ,Nanoemulsion (NE) and emulsifiable concentrate (EC) containing biodiesel waste of 20-100 ppm gave 30 -90 % mortality rates against Periplaneta americana (Order: Blattodea, Family: Blattidae) within 24 hours. The LD 50 (Lethal dose) values recorded for the microemulsion, nanoemulsion and emulsifiable concentrate were 6.7646,10.385 & 46.7289 with biodiesel waste as a solvent or carriers for above formulations. Keywords: Permethrin, Microemulsion, Nanoemulsion, Emulsifiable Concentrate, Hydrophilic-lipophilic Balance (HLB), Biodiesel waste, Periplaneta Americana Introduction A microemulsion is thermodynamically stable, and usually a transparent liquid solution. In pharmaceuticals, microemulsions are the objects of investigation in relation to drug delivery 2 , because of their advantages (thermodynamic stability, ease of preparation, transparency, low viscosity, considerable potential for solubilizing a variety of drugs). The biodiesel production in India utilizes two main crops, karanja (Pongamia pinnata) and jatropha (Jatropha curcas) as major source of non-edible oils. Production of biodiesel generates 10% biodiesel waste liquid by volume approximately i.e. to say 10 kg of crude glycerol is generated as a by-product for every 100 kg of biodiesel produced. Majority of industrial products employ only purified glycerol as a raw material, and therefore bio diesel waste liquid is often discarded as a waste product. This liquid biodiesel waste creates disposal problems and in future, liquid biodiesel waste is likely to be produced in large amounts, with detrimental effects on the environment. Hence, there is an urgent need to convert crude glycerol into more valuable products. (Patent filed, Application No.: 2391/MUM/2008). In our previous research work, biodiesel has been used as a solvent for oil soluble synthetic pyrethroid based conventional formulations like emulsifiable concentrate 7 . The formulations thus developed were having improved storage stability. In this paper we have used biodiesel waste in new generation water based liquid formulation of synthetic pyrethroids to improve physical as well as chemical stability of formulation as well as to increase its efficacy as compared to conventional or solvent based formulation. Experimental section Biodiesel waste (jatropha oil) from I.I.T Delhi, Permethrin (Technical, purity 93%) was purchased from United Phosphorous Limited, Mumbai, Polysorbate 80 (Tween 80) and Span 80 (sorbitan monooleate) from Merck India Limited. Test insect Cultures of Periplaneta americana were maintained at laboratory conditions, 60-70% RH, 27±1ºC and darkness i.e. 12:12 (L:D) h photocycle 8 . ATR-FTIR analysis Infrared (IR) spectra were recorded on a Bruker alpha ATR-FTIR spectrophotometer using the attenuated total reflectance (ATR) technique, and values are expressed as gmax cm_1. —————— *Author for Correspondence E-mail: drsaurabh_dubey@rediffmail.com