Copyright@ Shaukat Hayat, Farmanullah | Biomed J Sci & Tech Res | BJSTR. MS.ID.005225. 24836 Research Article ISSN: 2574 -1241 A Study on The Prevalence and Associated Microorganisms of Subclinical Mastitis in Buffaloes at Swat, Khyber Pakhtunkhwa Shaukat Hayat 1 *, Muhammad Faizul Hassan 2 , Farmanullah 3 *, Haider Hayat 4 , Tariq Khan 5 , Sardar Ali 1 , Muhammad Altaf Hussain 3 , Asma Babar 3 and Nadia Rahim 3 1 Livestock & Dairy Development Department, (Extension) Khyber Pakhtunkhwa, Pakistan 2 Veterinary Research & Disease Investigation Center, Pakistan 3 Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine, Sciences, Uthal, Pakistan 4 Department of Pathology, University of Veterinary and Animal Sciences, Pakistan 5 Department of Fisheries and Aquaculture, University of Veterinary & Animal Sciences, Pakistan *Corresponding author: Farmanullah, Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Balochistan, Pakistan DOI: 10.26717/BJSTR.2020.32.005225 ARTICLE INFO Abstract The present study was conducted to determine the prevalence rates and associated microorganism of the subclinical mastitis in buffaloes in Swat. Four areas were selected from collection of the data i.e. Khwazakhela, Kabal, Matta and Marghazar and 200 milk samples were collected from each site and were examined through SFMT for the presence of subclinical mastitis. The results of the study revealed that overall prevalence rates were found to be 44.5 % [highest in Kabal (50.0%), followed by Matta (48.0 %), Marghazar (42.0%) and Khwazakhela (38.0%)]. Out of total 800 quarters 183 were found to be infected (22.87%). The SFMT positive samples when cultured at VR&DIC, Balogram, Swat, revealed the isolation and identification of nine bacterial species: Staphy. aureus (35.51%), E.coli (16.39%), Strept. agalactea (14.75 %), Strept. dysgalactae (7.83%), Proteus vulgaris (4.37 %), Citrobacter sp. (4.18%), Strept. uberis (3.82 %), Bacillus cereus (3.27%), Pseudomonas aeruginosa (1.63 %) and mixed infection (8.25%). Keywords: Buffaloes; Microorganisms; Prevalence; Subclinical Mastitis; Swat Received: November 14, 2020 Published: December 02, 2020 Citation: Shaukat H, Muhammad Faizul H, Farmanullah, Haider H, Tariq K, et al., A Study on The Prevalence and Associat- ed Microorganisms of Subclinical Mastitis in Buffaloes at Swat, Khyber Pakhtunkh- wa. Biomed J Sci & Tech Res 32(2)-2020. BJSTR. MS.ID.005225. Introduction Livestock is an important sector of agriculture in Pakistan, which accounts for 50% of the agricultural value, about 11% in the total GDP, of the country. The role of livestock in rural economy can be realized from the fact that 30-35 million rural population is engaged in livestock raising, having household holdings of 2-3 cattle/buffalo and 5-6 sheep/goats per family, that help them to derive 30-40 percent of their income from it. Among the common animal diseases, mastitis is the most important infectious disease of dairy animals, causing significant financial losses worldwide and is a major cause of antibiotics use in dairy buffaloes [1]. Mastitis is the inflammation of the parenchymal cells of the mammary glands associated with microbial infections and physiological changes [2]. Mastitis has two types-clinical and subclinical mastitis. In the clinical mastitis all the five cardinal signs of inflammation can be observed while the subclinical mastitis has no obvious manifestation and thus can only be detected by the milk examination [3]. The prevalence rates of subclinical mastitis have been reported 3-40 times more than the clinical mastitis [4]. Keeping in view, its importance, the present study was conducted to determine the prevalence rates and associated microorganism of the subclinical mastitis in buffaloes in Swat.