Mycorrhizal Species Diversity in Salam Rhizosphere (Syzigium Polyanthum) in Ex-Sand Mining Land in Cipancur, Ciawigebang District, Kuningan Regency Nina Herlina*, Ai Nurlaila, Bambang Yudayana, Haidar Rahadian, M. Razan Farras Forest Faculty, Kuningan University, Kuningan, Indonesia {nina.herlina@uniku.ac.id} Abstract. Mining activities on ex-excavated land C cause critical land damage and will affect the ecosystem of the land. As one of the efforts to restore the condition of the former C excavated land is by reclamation or revegetation. The use of Arbuscular Mycorrhizal Fungi (AMF) is an alternative that can be developed with various benefits including increasing plant growth, increasing soil nutrient absorption, and increasing plant tolerance to extreme conditions. This study aims to identify the types of mycorrhizae in the rhizosphere of salam (Syzygium polyanthum) in the ex-sand mines of Cipancur Village, Kalimanggis District, Kuningan Regency. The identification and observation of AMF colonization was carried out at the Laboratory of the Faculty of Forestry, Universitas Kuningan. The materials used were soil and root samples under a Salam tree (Syzygium polyanthum) stand. Spore morphology was identified using INVAM (2008). The results showed that the number of spores found under stands of Salam trees (Syzygium polyanthum) from 16 sample plots was 1,949 spores consisting of 3 types of AMF, namely Glomus sp., Gigaspora sp., and Acaulosprora sp Keywords: Arbuscular Mycorrhizal Fungi; Ex-Dug C; Reclamation 1 Introduction Utilization of non-renewable natural resources through sand mining activities must be able to reduce the level of environmental damage for human welfare. Post-mining land damage begins with the destruction of the physical, biological, and chemical properties of the soil followed by changes in the shape and structure of the landscape [1]. The decrease in the quality of the topsoil on ex-mining land is characterized by damage to soil structure, accelerated erosion, excessive washing, soil compaction, decreased soil pH, accumulation of heavy metals in the soil, depletion of organic matter, decreased plant nutrients, and decreased exchange capacity. cations, decreased microbial activity [2]. Changes in the structure and function of the landscape, both in the form of natural events and human disturbances, will have an impact on changes in the composition of plant and animal species [3]. The return of the soil layer to the former post-mining excavation is not able to restore the land condition to the same as the condition before mining [4]. However, knowledge about functional soil microbial changes in land as a result of reclamation with vegetation succession is still inadequate [2]. UNISET 2021, December 02, Kuningan, Indonesia Copyright © 2022 EAI DOI 10.4108/eai.2-12-2021.2320202